Edinburgh Research Explorer

TMT generated proteomic identification and quantification of muscle and NMJ enriched fractions in mice and humans

Dataset

Date made available25 Oct 2017

Description

The information in this deposit should meet the Minimum Information About Proteomic Experiments (MIAPE – Taylor et al 2007 Nature Biotechnology) guidelines regarding proteomic data reporting. There are four files associated with this deposit: 1. "Human and mouse NMJ TMT Analysis Methodology_Data share.docx". A Microsoft Word file describing the methodology employed to generate the data shared here. 2. "Human and mouse NMJ TMT Analysis Results_Data share.xlsx". A Microsoft Excel file with the proteomic output from MaxQuant as described in the document above. This file contains two tabs. One is a summary of the protein information from human and mouse samples and one is the peptide information associated with those identifications. 3. "readme.txt". Documentation. 4. "Human_and_mouse_NMJ_TMT_Analysis_Methodology_DataShare.pdf". For accessibility, this PDF version of the methodology file (listed as number 1 above) is included.

Abstract

The neuromuscular junction (NMJ) plays a fundamental role in transferring information from lower motor neurons to skeletal muscle to generate movement. It is also an experimentally-accessible model synapse routinely studied in animal models to explore fundamental aspects of synaptic form and function. This dataset comprises part of a wider study examining the composition and form of the neuromuscular junction (NMJ) in mice and humans. The data sets demonstrate differential expression of core synaptic proteins and molecular pathways at the human NMJ. This data as part of a larger study reveals human-specific cellular and molecular features of the NMJ that distinguish them from comparable synapses in other mammalian species. Abbreviation: TMT – “Tandem Mass Tag multiplexing reagents” for labelling amine groups.

Data Citation

Wishart, Thomas. (2017). TMT generated proteomic identification and quantification of muscle and NMJ enriched fractions in mice and humans, [dataset]. http://dx.doi.org/10.7488/ds/2240

Access status

Open

ID: 45741869