Description
Production of genetically altered chickens in a Specified Pathogen Free (SPF) context: challenges and recent advancesAbstract:
The National Avian Research Facility (NARF) at the Roslin Institute is a large repository of conventional and Specified Pathogen Free (SPF) lines of inbred, outbred, and transgenic reporter chickens available to the international research community. NARF is also a leader in genetic engineering of the chicken genome. It has recently implemented a highly efficient pipeline for creating genetically altered (GA) chickens, capitalising on strategies developed in the McGrew lab, in particular the ability to precisely edit avian primordial germ cells (PGCs) [1] and the creation of the iCaspase9 sterile surrogate host [2]. Many new lines of GA chickens have already been created within NARF conventional unit using this pipeline.
To broaden this approach and offer the research community the opportunity to create GA birds of known health status, NARF has recently implemented this pipeline in its SPF unit, which houses unvaccinated flocks certified free from 17 specified pathogens. GA chickens created in this SPF context are particularly suitable for studying the immune system.
The challenges associated with transposing this gene-editing pipeline to a highly biosecure SPF unit, and the lines successfully created so far, will be discussed.
| Period | 8 Nov 2023 → 10 Nov 2023 |
|---|---|
| Event title | 12th European Symposium on Poultry Genetics |
| Event type | Symposium |
| Location | Hannover, GermanyShow on map |
| Degree of Recognition | International |