Avian Research Symposium 2021

Activity: Academic talk or presentation typesOral presentation


Creating genetically altered chickens in a SPF context: challenges and recent advance.

A highly efficient pipeline for the creation of genetically altered (GA) chickens using gene-edited primordial germ cells (PGCs) and bespoke sterile surrogate hosts has recently been established at the National Avian Research Facility (NARF). This pipeline, which relies on advanced strategies and tools developed in the McGrew lab, has already allowed the generation of several lines of GA chickens for the study of chicken development and immunity within the Greenwood building, the main NARF unit. To offer the research community the opportunity to create GA birds of known health status to study disease resistance or facilitate exchange of materials with research groups worldwide, this technology has also recently been introduced in the Bumstead building, the NARF Specified Pathogen Free (SPF) unit. The challenges associated with the establishment of a bio-secure pipeline for the creation of GA chickens in a SPF context, and the lines successfully created so far, will be discussed.
Period24 Feb 202125 Feb 2021
Held atThe Roslin Institute