Description
Genetic crossing were undertaken between the anthelmintic sensitive genome strain-MHco3 (ISE) and a multi-drug resistant strain-MHco18 (UGA) derived from southern USA. Day 14, one hundred female (MHco3) and 100 male (MHco18) and vice versa were harvested from donor sheep and surgically transferred to the abomasum of a recipient sheep. The eggs of F1 and F2 released from these worms were further incubated to form L3 stage and then used to orally infect another donor sheep to obtain F2 progeny treated with three broad spectrum anthelmintic drugs [Ivermectin (IVM), Benzimidazoles (BZ) and Levamisole (Lev)]. A panel of six microsatellite markers were used to monitor the success of genetic crossing procedure of F1 progeny. Microsatellite markers were also used to monitor the IVM treated F2 genetic crossing procedure based on the presence and absence strain specific alleles, there was one marker (Hcms 8a20) in the chromosome V, which alleles specific to parental resistance strain, was retained after IVM treatment give us a strong evidence of the linkage of IVM resistance conferring locus. We are further investigating with the panel of 17 microsatellite markers located in chromosome V from IVM drug selected F2 progeny to look for the detail evidence of genetic linkage to IVM resistance conferring locus providing a starting point for more detailed studies to identify the mutations linked to IVM resistance. Sensitivity of the BZ drug against F1 and F2 have been phenotypically and genotypically assessed using the egg hatch assay and pyrosequencing assay of known isotype-1 β tubulin SNPs.| Period | 2017 |
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| Event title | PARACON Pakistan |
| Event type | Conference |