Projects per year
Expression profiling by high throughput sequencing of ruminant (cattle and sheep) myeloid cells: 36 samples were profiled in total; derived from four biological replicates (i.e. isolated from four cattle or sheep). The myeloid subsets (of 4 individuals) were all derived from peripheral blood mononuclear cell preparations as follows:
Sheep: (a) monocytes - purified by CD14+ MACS separation; (b) monocyte derived dendritic cells - cultured CD14+ monocytes with ovine IL-4 & GMCSF; (c) monocyte derived macrophages (MDM) - cultured adherent cells; (d) Chlamydia infected MDM.
Cattle: (a) CD14+ monocytes - purified by CD14+ MACS separation; (b) CD16hi++ monocytes - purified by CD16+ MACS separation, followed by flow cytometry to obtain the CD16hi++ monocytes (c) monocyte derived dendritic cells - cultured CD14+ monocytes with bovine IL-4 & GMCSF; (d) monocyte derived macrophages (MDM) - cultured adherent cells; (e) Chlamydia infected MDM.
|Date made available||2 Oct 2018|
|Date of data production||2014 - 2015|
- 2 Finished
Collie, D., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Fitzgerald, R., Freeman, T., Gally, D., Gill, A., Glass, E., Hocking, P., Hope, J., Hume, D., Kaiser, P., Mabbott, N., McLachlan, G., Morrison, L., Stevens, J., Stevens, M. & Watson, M.
1/04/12 → 31/03/17
Glass, E., Hope, J. & Entrican, G.
1/02/12 → 31/07/15