2189N and 2190N Ruminant cattle myeloid cell subsets

Description
Expression profiling by high throughput sequencing of ruminant (cattle and sheep) myeloid cells: 36 samples were profiled in total; derived from four biological replicates (i.e. isolated from four cattle or sheep). The myeloid subsets (of 4 individuals) were all derived from peripheral blood mononuclear cell preparations as follows:
Sheep: (a) monocytes - purified by CD14+ MACS separation; (b) monocyte derived dendritic cells - cultured CD14+ monocytes with ovine IL-4 & GMCSF; (c) monocyte derived macrophages (MDM) - cultured adherent cells; (d) Chlamydia infected MDM.
Cattle: (a) CD14+ monocytes - purified by CD14+ MACS separation; (b) CD16hi++ monocytes - purified by CD16+ MACS separation, followed by flow cytometry to obtain the CD16hi++ monocytes (c) monocyte derived dendritic cells - cultured CD14+ monocytes with bovine IL-4 & GMCSF; (d) monocyte derived macrophages (MDM) - cultured adherent cells; (e) Chlamydia infected MDM.

Myeloid subsets have distinct immunological functions. We had already phenotypically characterised unstimulated subsets in cattle and sheep. RNA-seq was conducted to provide additional and novel information. Although closely related, cattle and sheep differ in their susceptibility to a number of pathogens including Chlamydia abortus. We also investigated differences between cattle and sheep monocyte derived macrophages in their interaction with Chlamydia abortus.

Glass, E; Hope, J; Entrican, G; Corripio-Miyar, Y; Doull LE "2189N and 2190N Ruminant cattle myeloid cell subsets" Edinburgh DataVault DOI: 10.7488/775660a6-2d55-4416-979e-0d9e597787ea