Description
Expression profiling by high throughput sequencing of ruminant (cattle and sheep) myeloid cells: 36 samples were profiled in total; derived from four biological replicates (i.e. isolated from four cattle or sheep). The myeloid subsets (of 4 individuals) were all derived from peripheral blood mononuclear cell preparations as follows:
Sheep: (a) monocytes - purified by CD14+ MACS separation; (b) monocyte derived dendritic cells - cultured CD14+ monocytes with ovine IL-4 & GMCSF; (c) monocyte derived macrophages (MDM) - cultured adherent cells; (d) Chlamydia infected MDM.
Cattle: (a) CD14+ monocytes - purified by CD14+ MACS separation; (b) CD16hi++ monocytes - purified by CD16+ MACS separation, followed by flow cytometry to obtain the CD16hi++ monocytes (c) monocyte derived dendritic cells - cultured CD14+ monocytes with bovine IL-4 & GMCSF; (d) monocyte derived macrophages (MDM) - cultured adherent cells; (e) Chlamydia infected MDM.
Abstract
Data Citation
| Date made available | 2 Oct 2018 |
|---|---|
| Publisher | Edinburgh DataVault |
| Date of data production | 2014 - 2015 |
Projects
- 2 Finished
-
Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research
-
the route to identification of the immunological correlates of protection in ruminants
Glass, E. (Principal Investigator), Hope, J. (Co-investigator) & Entrican, G. (Researcher)
1/02/12 → 31/07/15
Project: Research
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