Abstract
Live image data used in the study "An in vitro - agent-based modelling approach to optimisation of culture
medium for generating muscle cells".
Live imaging was performed using an inverted microscope (Zeiss Cell Observer SD or Zeiss Cell observer) in widefield mode, using a 20x phase air objective (Plan-Apochromat Ph2 NA 0.80 or EC Plan-Neofluar Ph 2 NA 0.50, respectively).
Cells were imaged 2 hours after adding the respective differentiation medium.
3x3 tiles with 10% overlap with 2x2 binning were taken every 5 minutes for a minimum of 12 hours.
Images were stitched using Zen Blue software and stacked into 30 minutes videos for further quantitative analysis.ions.
medium for generating muscle cells".
Live imaging was performed using an inverted microscope (Zeiss Cell Observer SD or Zeiss Cell observer) in widefield mode, using a 20x phase air objective (Plan-Apochromat Ph2 NA 0.80 or EC Plan-Neofluar Ph 2 NA 0.50, respectively).
Cells were imaged 2 hours after adding the respective differentiation medium.
3x3 tiles with 10% overlap with 2x2 binning were taken every 5 minutes for a minimum of 12 hours.
Images were stitched using Zen Blue software and stacked into 30 minutes videos for further quantitative analysis.ions.
Data Citation
Hardman, David. (2022). An in vitro - agent-based modelling approach to optimisation of culture medium for generating muscle cells: Live images, [moving image]. University of Edinburgh. Usher Institute. https://doi.org/10.7488/ds/3435.
| Date made available | 11 Apr 2022 |
|---|---|
| Publisher | Edinburgh DataShare |