Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 retains functional activity.
Brewster, Richard C; Gavins, Georgina C; Günthardt, Barbara; Farr, Sarah; Webb, Kimberly M; Voigt, Philipp; Hulme, Alison N. (2016). Chloromethyl-triazole: A new motif for site-selective pseudo-acylation of proteins, [dataset]. University of Edinburgh. School of Chemistry. http://dx.doi.org/10.7488/ds/1484.