Description
NASCARRAYS-108; GEO GSE5612
Abstract
Our aim is to study the circadian expression of genes to aid in our attempt of modelling the Arabidopsis circadian clock. Circadian microarray data have previously been published for plants after white light (WL)-dark cycles, using the 8k chip (Harmer et al. 2000). We intend to repeat this experiment using the 26k chips and are coordinating with Dr. Harmer, who is pursuing complementary experiments in UC Davis. Plants will be transferred to continuous WL after entrainment to 12h:12h light dark cycles. RNAs will be harvested every 4 hours over two days, with the same accession and sampling intervals used previously by Harmer et al. The two days of sampling provide internal replication. Our experience shows that this is the most economical design: it is easier to identify rhythms over a two-day timecourse than in two replicates of a single day. Hence: 13 RNA samples on 13 chips in total.
| Date made available | 2005 |
|---|---|
| Publisher | University of Edinburgh |
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Using higher-order dynamic Bayesian networks to model periodic data from the circadian clock of Arabidopsis Thaliana.
Daly, R., Edwards, K. D., O'Neill, J. S., Aitken, S., Millar, A. & Girolami, M., 2009, Pattern Recognition in Bioinformatics: 4th IAPR International Conference, PRIB 2009, Sheffield, UK, September 7-9, 2009. Proceedings. Springer, p. 67-78 12 p. (Lecture Notes in Computer Science; vol. 5780).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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Modelling non-stationary gene regulatory processes with a non-homogeneous Bayesian network and the allocation sampler
Grzegorczyk, M., Husmeier, D., Edwards, K. D., Ghazal, P. & Millar, A. J., 15 Sept 2008, In: Bioinformatics. 24, 18, p. 2071-2078 8 p.Research output: Contribution to journal › Article › peer-review
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FLOWERING LOCUS C mediates natural variation in the high-temperature response of the Arabidopsis circadian clock
Edwards, K. D., Anderson, P. E., Hall, A., Salathia, N. S., Locke, J. C. W., Lynn, J. R., Straume, M., Smith, J. Q. & Millar, A. J., 2006, In: Plant Cell. 18, 3, p. 639-650 12 p.Research output: Contribution to journal › Article › peer-review
Projects
- 1 Finished
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Modelling circadian gene regulation in Arabidopsis thaliana
Millar, A. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/03/05 → 30/09/06
Project: Research
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