Code for the analysis of scRNA-seq data from spinal cord ependymal cells of young and aged mice

The adult spinal cord stem cell potential resides within the ependymal cell population and declines with age. Ependymal cells are, however, heterogeneous and the biological diversity this represents and how it changes with age remain unknown. This repository contains the code used to analyse single-cell RNA sequencing data of spinal cord ependymal cells from adult and aged mice that led us to uncover the profiles of all known ependymal cell subtypes and new immature and mature cell states. The R scripts are numbered based on which figure the analyses relate to. The preprint of the study can be found at: https://www.biorxiv.org/content/10.1101/2022.03.07.483249v1 Raw sequencing data, raw count matrices, and processed data have been deposited at ArrayExpress under accession numbers E-MTAB-11561 (10x Genomics) and E-MTAB-11563 (Smart-seq2).

Rodrigo Albors, A., Singer, G. A., Llorens-Bobadilla, E., Frisén, J., May, A. P., Ponting, C. P., & Storey, K. G. (2022). Code for the analysis of scRNA-seq data from spinal cord ependymal cells of young and aged mice (v1.0.0). Zenodo. https://doi.org/10.5281/zenodo.7459071