Data associated with the manuscript. "Electrochemical sensing of human neutrophil elastase and polymorphonuclear neutrophil activity". Design and evaluation of a peptide-based electrochemical biosensor for HNE activity detection. The analytical characteristics of the sensor were evaluated. The platform was applied for the detection of HNE in real blood samples. Dataset includes set of tables containing the data used for obtaining figures and results.
Human neutrophil elastase (HNE) is a serine protease, produced by polymorphonuclear neutrophils (PMNs), whose uncontrolled production has been associated with various inflammatory disease states as well as tumour proliferation and metastasis. Here we report the development and characterisation of an electrochemical peptide-based biosensor, which enables the detection of clinically relevant levels of HNE. The sensing platform was characterised in terms of its analytical performance, enzymatic cleavage kinetics and cross-reactivity and applied to the quantitative detection of protease activity from PMNs from human blood.
González-Fernández, Eva; Staderini, Matteo; Yussof, Amirah; Scholefield, Emma; Murray, Alan F; Mount, Andrew R; Bradley, Mark. (2018). Data associated with manuscript: Electrochemical sensing of human neutrophil elastase and polymorphonuclear neutrophil activity, [dataset]. University of Edinburgh. School of Chemistry. School of Engineering. http://dx.doi.org/10.7488/ds/2409.