Provides URLs to the Data, Code and Models for Flis et al. RS Open Biology 2015 article entitled, "Defining the robust behaviour of the plant clock gene circuit with absolute RNA timeseries and open infrastructure".
Our understanding of the complex, transcriptional feedback loops in the circadian clock mechanism has depended upon quantitative, timeseries data from disparate sources. We measure clock gene RNA profiles in Arabidopsis thaliana seedlings, grown with or without exogenous sucrose, or in soil-grown plants and in wild-type and mutant backgrounds. The RNA profiles were strikingly robust across the experimental conditions, so current mathematical models are likely to be broadly applicable in leaf tissue. In addition to providing reference data, unexpected behaviours included co-expression of PRR9 and ELF4, and regulation of PRR5 by GI. Absolute RNA quantification revealed low levels of PRR9 transcripts (peak ~50 copies/cell) compared with other clock genes, and three-fold higher levels of LHY RNA (>1500 copies/cell) than of its close relative CCA1. The data are disseminated from BioDare, an online repository for focussed timeseries data, which is expected to benefit mechanistic modelling. One data subset successfully constrained clock gene expression in a complex model, using publicly-available software on parallel computers, without expert tuning or programming. We outline the empirical and mathematical justification for data aggregation in understanding highly-interconnected, dynamic networks such as the clock, and the observed design constraints on the resources required to make this approach widely accessible.
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