Data for Comparison of two lines of tauGFP transgenic mice designed for lineage tracing

Dataset

Abstract

The data are numerical data used to produce some figures and tables for a manuscript comparing two lines of tauGFP transgenic mice designed for lineage tracing.
Study Abstract: The tauGFP reporter fusion protein is produced nearly ubiquitously by the TgTP6.3 transgene in TP6.3 mice and its localisation to microtubules offers some advantages over soluble GFP as a lineage marker. However, TgTP6.3Tg/Tg homozygotes are not viable and TgTP6.3Tg/- hemizygotes are smaller than wild-type. TP6.4 mice carry the TgTP6.4 transgene, which was produced with the same construct used to generate TgTP6.3, so we investigated whether TgTP6.4 had any advantages over TgTP6.3. Although TgTP6.4Tg/Tg homozygotes died before weaning, TgTP6.4Tg/- hemizygotes were viable and fertile and only males were significantly lighter than wild-type. The TgTP6.4 transgene produced the tauGFP fusion protein by the 2-cell stage and it was widely expressed in adults but tauGFP fluorescence was weak or absent in several tissues, including some neural tissues. The TgTP6.4 transgene expression pattern changed over several years of breeding and mosaic transgene expression became increasingly common in all expressing tissues. Although mosaic expression makes the TgTP6.4 transgene unsuitable for use as a conventional cell lineage marker, mosaicism provides a useful system for visualising clonal lineages that arise during development or maintenance of adult tissues. Lineages in the adrenal cortex of TgTP6.4Tg/- hemizygotes were qualitatively and quantitatively comparable to lineages reported previously for other mosaic transgenic mice, X-inactivation mosaics and chimaeras. Mosaicism occurred less frequently in TP6.3 than TP6.4 mice and was only observed in the corneal epithelium and adrenal cortex. Such differences in the occurrence of mosaicism between related transgenic lines might provide a useful system for investigating the mechanism of transgene silencing.

Data Citation

Sharp, Linda; Pratt, Thomas; MacKay, Gillian E.; Keighren, Margaret A.; Flockhart, Jean H.; Chandler, Emma J.; Price, David J.; Mason, John O.; West, John D.. (2017). Data for Comparison of two lines of tauGFP transgenic mice designed for lineage tracing, [dataset]. University of Edinburgh, Edinburgh Medical School. http://dx.doi.org/10.7488/ds/2049
Date made available30 May 2017
PublisherEdinburgh DataShare

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