Data from characterisation of 3D printed devices for the separation of plasma from whole blood samples. These are simple, low cost devices using a commercially available filtration membrane which can extract plasma from a 50 micro-litre capillary blood sample in less than two minutes and are designed to enable quick and easy sample preparation for point of care health testing. The data set is taken from seven different designs of devices using samples of horse blood obtained from the Easter Bush Pathology Laboratory at the University. The data includes full details of the input samples, the plasma output yield and quality, as well as the failure rate of the devices. Full information on the data set can be found in the readme file. The data was used in the publication: https://doi.org/10.3390/mi15030359 and the PhD thesis: “Development of microfluidic devices for the separation of blood plasma from capillary samples” http://dx.doi.org/10.7488/era/3215