Dataset related to article: "Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies"

This record contains raw data related to article "Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies" Abstract: Glioblastoma is invariably lethal and responds poorly to immune checkpoint blockade. Here, we examined the impact of regulatory T (Treg) cell depletion on glioblastoma progression and immunotherapy responsiveness. In human glioblastoma, elevated Treg cell signatures correlated with poorer survival outcomes, with these cells expressing high CD25 levels. In Nf1⁻/⁻Pten⁻/⁻EGFRvIII⁺ glioblastoma-bearing mice, a single dose of non-interleukin-2 (IL-2) blocking (NIB) anti-CD25 antibodies depleted Treg cells and promoted CD8⁺ T cell clonal expansion and partial tumor control, further enhanced by PD1-blockade. Treg cell depletion induced interferon-γ-dependent tumor microenvironment remodeling, increasing Fcγ receptor expression on intratumoral myeloid cells and enhancing phagocytosis. Combining anti-CD25ⁿⁱᵇ with anti-EGFRvIII tumor-targeting antibodies resulted in complete tumor control. Anti-human CD25ⁿⁱᵇ treatment of glioblastoma patient-derived tumor fragments effectively depleted Treg cells and activated CD8⁺ T cells. These findings underscore the therapeutic relevance of Treg targeting in glioblastoma and unveil potent combination strategies for anti-CD25ⁿⁱᵇ based on innate cell activation.

Galvez-Cancino, F., Navarrete, M., Beattie, G., Puccio, S., Conde-Gallastegi, E., Foster, K., Morris, Y., Sahwangarrom, T., Karagianni, D., Liu, J., Lee, A. J. X., Garyfallos, D., Simpson, A. P., Mastrokalos, G.-T., Nannini, F., Costoya, C., Anantharam, V., Cianciotti, B. C., Bradley, L., … Quezada, S. A. (2025). Dataset related to article: "Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies" [Data set]. Zenodo. https://doi.org/10.5281/zenodo.15240316