Gene expression in microdissected embryonic regions during mouse axis elongation

  • Filip Wymeersch (RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo) (Creator)
  • Stavroula Skylaki (ETH Zürich) (Creator)
  • Yali Huang (Creator)
  • Julia Watson (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU) (Creator)
  • Constantinos Economou (Creator)
  • Carylyn Marek-Johnston (Creator)
  • Simon Tomlinson (Creator)
  • Valerie Wilson (Creator)

Dataset

Description

Experiment type: Expression profiling by array

Summary: This dataset contains spatiotemporal transcriptome information on different axial progenitors throughout mouse axis elongation. Neuromesodermal Progenitors (NMPs), Lateral and Paraxial Mesoderm Progenitors (LPMPs), and Notochord Progenitors (NotoPs) show distinct expression profiles. Extensive similarity exist between NMPs and their immediate mesoderm-committed descendants at each stage investigated. Over time transcriptional changes occur in LPMPs and NMPs, with the major change occurring in NMPs between early somitogenesis and completion of trunk morphogenesis. In contrast, NotoPs contain a more stable transcriptome over time.

Overall design: Total RNA was obtained from microdissected, embryonic regions from wildtype mouse embryos at different embryonic stages during axis elongation. Regions of a single type were pooled to constitute one sample.

Data Citation

Wymeersch FJ, Skylaki S, Huang Y, Watson JA, Economou C, Marek-Johnston C, Tomlinson SR, Wilson V, 2018, Gene expression in microdissected embryonic regions during mouse axis elongation, GEO, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE120870

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