Time-series data for the FY4 prototrophic strain of budding yeast growing in different concentrations of fructose in minimal media in a Tecan plate reader after pre-growth in SC medium with 2% pyruvate. The dataset relates to the publication 'Nunchaku: Optimally partitioning data into piece-wise linear segments' by Yu Huo, Hongpei Li, Xiao Wang, Xiaochen Du, Peter S. Swain (https://doi.org/10.1101/2023.05.26.542406).