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Rat embryonic stem cells exhibit basal levels of spontaneous differentiation in 2i+LIF (2iL) culture. Expression of a stem cell specific Rex1-EGFP knock-in reporter transgene in rat ESCs was used to isolate functionally distinct subsets of cells in rat ESC cultures, and determine their transcriptional profiles by single cell RNA sequencing.
Burdon, Tom; Meek, Stephen; Chandra, Tamir; Joshi, Anagha. (2019). Primary Dataset of Single cell transcriptional profiling of rat embryonic stem cells defined by Rex1-EGFP reporter expression, [dataset]. University of Edinburgh. Roslin Institute. https://doi.org/10.7488/078deee1-6e1e-4bae-a483-4ec093ec126f .