Processed proteomic and phosphoproteomic timeseries from Ostreococcus tauri, with Gene Ontology enrichment, from "A phospho-dawn of protein modification anticipates light onset in the picoeukaryote O. tauri"

Diel regulation of protein levels and protein modification had been less studied than transcript rhythms. These data tables in .XLSX format report partial proteome (Table_S1) and phosphoproteome data (Table_S2), assayed using shotgun mass-spectrometry, from cultures of the alga Ostreococcus tauri under light-dark cycles, sampled at Zeitgeber times (ZT, hours) 0, 4, 8, 12, 16 and 20. 10% of quantified proteins but two-thirds of phosphoproteins were rhythmic. Gene Ontology enrichment analysis was applied to infer the functional enrichment of the proteins or phosphoproteins, grouped by their loadings in PCA analysis (Table_S3), by hierarchical clustering (Table_S4) or by the peak time of their rhythmic profile (Table_S5).Prompted by night-peaking and apparently dark-stable proteins, we also tested the proteome of cultures transferred to prolonged darkness for 24, 48, 72 or 96h (Table_S6), where the proteome changed less than under the diel cycle. The raw data are available from ProteomeXchange, with identifiers PXD001734, PXD001735 and PXD002909.

Hindle, Matthew M., Martin, Sarah F., Noordally, Zeenat, Millar, Andrew J., Simpson, T. Ian, & Le Bihan, Thierry. (2023). Processed proteomic and phosphoproteomic timeseries from Ostreococcus tauri, with Gene Ontology enrichment, from "A phospho-dawn of protein modification anticipates light onset in the picoeukaryote O. tauri" [Data set]. Zenodo. https://doi.org/10.5281/zenodo.7742118