RNA-sequencing of fetal thymic epithelial cells (TECs), in control, loss-of-function and gain-of-function Notch mutants

Assay the transcriptomes of E12.5 control and Rbpj conditional knockout TECs and E14.5 control, Rbpj knockout and Rosa26NICD TECs. 100 cells per sample.

We have shown that Notch signalling pathway is a potent regulator of thymic epithelial progenitor cell differentiation. Here we generated RNA-sequencing profile of early fetal TECs expressing low, normal or high levels of Notch. We found that loss of Notch function has no significant effects on the E12.5 TEC transcriptome. In contrast, loss and gain-of-function of Notch result in considerable changes in the E14.5 TEC transcriptome. This study uncovered a role of Notch in regulating cell state in TEC differentiation, as well as target genes and pathways regulated by Notch in this context.