Role of Sox2 in preimplantation and postimplantation pluripotency

Andrea Corsinotti (Creator)
Frederick C K Wong (Creator)
Tulin Tatar (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland.) (Creator)
Iwona Szczerbinska (Creator)
Florian Halbritter (Creator)
Douglas Colby (Creator)
Sabine Gogolok (Creator)
Raphael Pantier (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland.) (Creator)
Kirsten Liggat (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland.) (Creator)
Elham S Mirfazeli (Creator)
Elisa Hall-Ponsele (Creator)
Nicholas Mullin (Creator)
Valerie Wilson (Creator)
Ian Chambers (Creator)

Dataset

Description

Microarray gene expression analysis of mouse embryonic stem cells in different culture conditions. 4 conditions: AFN=Activin/FGF/N2B27 (unsorted (All) or sorted by Sox2 expression into High or Low), PCLN=LIF/2i/N2B27, LBN=LIF/BMP/N2B27 (sorted by Sox2 expression into High and Low) and FCSL=LIF/FCS/GMEM (sorted by Sox2 expression into High and Low). 3 replicates per group. 24 samples total.

Abstract

Sox2 is a key transcription factor directing embryonic stem cell (ESC) pluripotency. However, the role of SoxB1 proteins in epiblast stem cell (EpiSC) pluripotency is unknown. Here we compare the transcriptomes of pluripotent cells cultured in different conditions that express different levels of Sox2. While highest Sox2 level in ESCs are enriched for naive pluripotency markers, ESCs and EpiSCs expressing the same Sox2 level were transcriptionally distinct. Thus Sox2 levels do not dictate the distinction between ESC and EpiSC states.

Data Citation

Corsinotti A, Wong FC, Tatar T, Szczerbinska I et al. Distinct SoxB1 networks are required for naïve and primed pluripotency. Elife 2017 Dec 19;6. PMID: 29256862

Date made available	19 Dec 2017
Publisher	National Center for Biotechnology Information (Gene Expression Omnibus)

Access Dataset

Dataset publicly available at the NCBI Gene Expression Omnibus (accession no: GSE99185)

1 Article

Distinct SoxB1 networks are required for naïve and primed pluripotency
Corsinotti, A., Wong, F. C. K., Tatar, T., Szczerbinska, I., Halbritter, F., Colby, D., Gogolok, S., Pantier, R., Liggat, K., Mirfazeli, E. S., Hall-Ponsele, E., Mullin, N. P., Wilson, V. & Chambers, I., 19 Dec 2017, In: eLIFE. 6, 28 p., e27746.
Research output: Contribution to journal › Article › peer-review

Open Access
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Cite this

Corsinotti, A. (Creator), Wong, F. C. K. (Creator), Tatar, T. (Creator), Szczerbinska, I. (Creator), Halbritter, F. (Creator), Colby, D. (Creator), Gogolok, S. (Creator), Pantier, R. (Creator), Liggat, K. (Creator), Mirfazeli, E. S. (Creator), Hall-Ponsele, E. (Creator), Mullin, N. (Creator), Wilson, V. (Creator), Chambers, I. (Creator) (19 Dec 2017). Role of Sox2 in preimplantation and postimplantation pluripotency. National Center for Biotechnology Information (Gene Expression Omnibus).