SHARPER-DOSY: Sensitivity Enhanced Diffusion-Ordered NMR Spectroscopy

A liquid-state NMR method is reported that increases the sensitivity of the existing techniques for the measurement of diffusion coefficients of pure compounds 10-100 times. The associated 100-10000-fold time saving is achieved by removing the chemical shift separation of 1H resonances and their splitting due to J couplings. Diffusion coefficients are measured from a very narrow singlet, which is invariant to magnetic field inhomogeneity, and is obtained through signal acquisition embedded in short (< 0.5 ms) spin-echo intervals separated by non-selective 180° or 90° pulses. The proposed experiment incorporates signal selection and suppression of unwanted signals, including those of the solvent, and makes it possible to measure diffusion coefficient using as little as few hundred of nanograms of a medium-size organic molecule on high field cryoprobe NMR spectrometers in a matter of minutes. This archive contains the original data presented in the associated manuscript. The dataset relates to the upcoming publication George Peat, Patrick J. Boaler, Claire L. Dickson, Guy C. Lloyd-Jones, Dušan Uhrín (2023), "SHARPER-DOSY: Sensitivity Enhanced Diffusion-Ordered NMR Spectroscopy". Nature communications (accepted for publication).