Rat embryonic stem cells exhibit basal levels of spontaneous differentiation in 2i+LIF (2iL) culture. Expression of a stem cell specific Rex1-EGFP knock-in reporter transgene in rat ESCs was used to isolate functionally distinct subsets of cells in rat ESC cultures, and determine their transcriptional profiles by single cell RNA sequencing.
Burdon, Tom; Meek, Stephen; Joshi, Anagha; Chandra, Tamir. (2019). Single cell transcriptional profiling of rat embryonic stem cells defined by Rex1-EGFP reporter expression, [dataset]. University of Edinburgh. Roslin Institute. https://doi.org/10.7488/ds/2639.