Abstract
We used luciferase reporter gene imaging to monitor circadian rhythms in leaves of Arabidopsis thaliana plants, achieving resolution close to the cellular level. Leaves grown without environmental cycles for up to 3 wk reproducibly showed spatiotemporal waves of gene expression consistent with intercellular coupling, using several reporter genes.
This dataset contains:
1) Experiments performed by Benedicte Wenden:
- 35S_LL_120310
- CCA1_LDgrownLLimaged_180310 - CCA1_LL_040210
- CCA1_LL_251109 - CCR2_LL_300610 - TOC1_LL_140710
2) Experiments performed by Sarah Hodge after the publication of Wenden et al., 2012:
- 2012-CCA1-Sarah_Hodge-good_quality
- 2012-CCA1-Sarah_Hodge-med_quality
This dataset contains:
1) Experiments performed by Benedicte Wenden:
- 35S_LL_120310
- CCA1_LDgrownLLimaged_180310 - CCA1_LL_040210
- CCA1_LL_251109 - CCR2_LL_300610 - TOC1_LL_140710
2) Experiments performed by Sarah Hodge after the publication of Wenden et al., 2012:
- 2012-CCA1-Sarah_Hodge-good_quality
- 2012-CCA1-Sarah_Hodge-med_quality
Data Citation
Millar, Andrew; Wenden, Benedicte; Hodge, Sarah. (2021). Spatiotemporal clock patterns in the leaf blade, [dataset]. University of Edinburgh. School of Biological Sciences. SynthSys.. https://doi.org/10.7488/ds/3030.
| Date made available | 4 May 2021 |
|---|---|
| Publisher | Edinburgh DataShare |
Research output
- 1 Article
-
Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf
Wenden, B., Toner, D. L. K., Hodge, S. K., Grima, R. & Millar, A. J., Apr 2012, In: Proceedings of the National Academy of Sciences (PNAS). 109, 17, p. 6757-6762Research output: Contribution to journal › Article › peer-review
Open AccessFile
Projects
- 2 Finished
-
TiMet: Linking the clock to metabolism
Millar, A. (Principal Investigator)
1/03/10 → 28/02/15
Project: Research
-
SynthSys; formerly CSBE: Centre for Systems Biology at Edinburgh
Millar, A. (Principal Investigator), Beggs, J. (Co-investigator), Ghazal, P. (Co-investigator), Goryanin, I. (Co-investigator), Hillston, J. (Co-investigator), Plotkin, G. (Co-investigator), Tollervey, D. (Co-investigator), Walton, A. (Co-investigator) & Robertson, K. (Researcher)
Biotechnology and Biological Sciences Research Council
8/01/07 → 31/12/12
Project: Research
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