Standard Operating Procedures (SOPs) for Senescence and Mitochondrial Function Assays in Cellular Models of Parkinson's Disease and Ageing

  • Alexander G Bury (Creator)
  • Alicja Olejnik (Creator)
  • Chiara Tocco (Creator)
  • Nathalie Saurat (Creator)
  • Dirk Hockemeyer (Creator)
  • Jens C. Schwamborn (Creator)
  • Lorenz Studer (Creator)
  • Pier Giorgio Mastroberardino (Creator)
  • Silvia Bolognin (Creator)
  • Tilo Kunath (Creator)
  • Viktor I Korolchuk (Creator)
  • Janelle Drouin-Ouellet (Creator)
  • Heather Mortiboys (Creator)

Dataset

Description

This collection comprises Standard Operating Procedures (SOPs) developed by the PD-AGE network for assessing key cellular processes relevant to Parkinson's disease (PD) and ageing. These SOPs cover assays for: Senescence and Inflammaging: β-Galactosidase activity, γ-H2AX foci detection, Senescence-Associated Secretory Phenotype (SASP) factor measurement (e.g., IL-6, IL-8), P16 and P21 protein detection, telomere length measurement, HMGB1 detection, and Lamin B1 detection. Mitochondrial Function: Mitochondrial DNA (mtDNA) damage detection, mitochondrial morphology analysis, and mitochondrial respiration measurement. These SOPs are designed to promote methodological rigour, consistency, and reproducibility across research groups investigating the interplay between ageing and PD using cellular models.

Subtitle (English)
Mitochondrial Respiration Measuring in Adherent Patient-Derived Cells
Subtitle (English)
Mitochondrial DNA (mtDNA) Mutation Detection - quantitative Polymerase chain Reaction (qPCR)
Subtitle (English)
Mitochondrial Morphology Analysis via High-Content Imaging
Subtitle (English)
Assessment of cell cycle-arresting proteins P21 and P16 by western blot
Subtitle (English)
Detection of Senescence-Associated β Galactosidase in cultured cells
Subtitle (English)
Immunocytochemical detection of γH2AX DNA damage foci in fixed cells
Subtitle (English)
Detection of the Senescence-Associated Secretory Phenotype (SASP)
Subtitle (English)
Senescent markers immunocytochemistry (HMGB1, Lamin A, Lamin B1, p16INK4a) in fixed cells
Subtitle (English)
Southern blot-based Telomere Restriction Fragment Assay

Data Citation

Bury, A. G., Olejnik, A., Tocco, C., Saurat, N., Hockemeyer, D., Schwamborn, J. C., Studer, L., Giorgio Mastroberardino, P., Bolognin, S., Kunath, T., Korolchuk, V. I., Drouin-Ouellet, J., & Mortiboys, H. (2025). Standard Operating Procedures (SOPs) for Senescence and Mitochondrial Function Assays in Cellular Models of Parkinson's Disease and Ageing. Zenodo. https://doi.org/10.5281/zenodo.15056603
Date made available20 Mar 2025
PublisherZenodo

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