Description
Bone Marrow derived macrophages (BMDM) were grown using L929 conditioned medium. On day 7, BMDM were mock-treated (16x 15cm dishes) or treated (16x 15cm dishes) with 10U/ml interferon gamma at time zero. 4-thiouridine (4sU) was then used to label newly transcribed RNA every 30 minutes for 8h (0 to 30, 30 to 60 etc. to give 16 discrete successive labellings) in mock and interferon gamma-treated samples. 16x Mock and 16x treated total samples were then isolated (32 in total) and newly transcribed RNA isolated from each total RNA sample using a protocol described in Dolken et al (PMID: 18658122). For analysis, 32 (16x mock and 16x treated) newly-transcribed RNA samples (every 30 minutes for complete 8h time course) and 16 (8x mock and 8x treated) total RNA samples (every hour for 8h time course) were hybridized to Affymetrix GeneST v1.0 arrays.
Abstract
Interferon gamma treatment of macrophages results in hundreds if not thousands of alterations in gene expression and an antiviral state being established in these cells. Little is known about relationship between transcript synthesis, abundance and decay in macrophages during the first hours after interferon gamma treatment and how these factors influence the antiviral cellular phenotype.
Microarrays were used to analyse alterations in transcript synthesis and abundance in BMDM treated with Interferon Gamma for 8h.
Microarrays were used to analyse alterations in transcript synthesis and abundance in BMDM treated with Interferon Gamma for 8h.
Data Citation
Robertson KA, Ghazal P (Division of Infection and Pathway Medicine, University of Edinburgh)
| Date made available | 7 Mar 2016 |
|---|---|
| Publisher | National Center for Biotechnology Information (Gene Expression Omnibus) |
| Temporal coverage | 1 Jun 2009 - 1 Jan 2010 |
| Date of data production | 2010 |
Research output
- 4 Article
-
An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway
Robertson, K., Hsieh, W. Y., Forster, T., Blanc, M., Lu, H., Crick, P. J., Yutuc, E., Watterson, S., Martin, K., Griffiths, S., Enright, A. J., Yamamoto, M., Madapura Marulasiddappa, P., Lennox, K. A., Behlke, M. A., Talbot, S., Haas, J., Dölken, L., Griffiths, W. J. & Wang, Y. & 2 others, , 3 Mar 2016, In: PLoS Biology. 14, 3, e1002364.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Rapid proteasomal elimination of 3-hydroxy-3-methylglutaryl-CoA reductase by interferon-γ in primary macrophages requires endogenous 25-hydroxycholesterol synthesis
Lu, H., Talbot, S., Robertson, K. A., Watterson, S., Forster, T., Roy, D. & Ghazal, P., Jul 2015, In: Steroids. 99, Pt B, p. 219-29 11 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
The Transcription Factor STAT-1 Couples Macrophage Synthesis of 25-Hydroxycholesterol to the Interferon Antiviral Response
Blanc, M., Hsieh, W. Y., Robertson, K. A., Kropp, K. A., Forster, T., Shui, G., Lacaze, P., Watterson, S., Griffiths, S. J., Spann, N. J., Meljon, A., Talbot, S., Krishnan, K., Covey, D. F., Wenk, M. R., Craigon, M., Ruzsics, Z., Haas, J., Angulo, A. & Griffiths, W. J. & 3 others, , 24 Jan 2013, In: Immunity. 38, 1, p. 106–118Research output: Contribution to journal › Article › peer-review
Open AccessFile
Projects
- 2 Finished
-
MicroRNA coupling of the sterol metabolic network to the antiviral immune response
Ghazal, P. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/07/13 → 31/08/16
Project: Research
-
SynthSys; formerly CSBE: Centre for Systems Biology at Edinburgh
Millar, A. (Principal Investigator), Beggs, J. (Co-investigator), Ghazal, P. (Co-investigator), Goryanin, I. (Co-investigator), Hillston, J. (Co-investigator), Plotkin, G. (Co-investigator), Tollervey, D. (Co-investigator), Walton, A. (Co-investigator) & Robertson, K. (Researcher)
Biotechnology and Biological Sciences Research Council
8/01/07 → 31/12/12
Project: Research
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