Research output per year
Research output per year
Personal profile
Current Research Interests
Biomolecular Mass Spectrometry, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), Top-Down Mass Spectrometry, Protein Chemistry, Enzymology, Structural Biology.
Biography
The core research theme of our group is the application of modern techniques in mass spectrometry (MS) for the functional and structural characterisation of biological systems. The School of Chemistry houses an excellent array of MS instrumentation - including FT-ICR, an ion mobility equipped Q-Tof, an extended mass range ESI Q-tof, and a MALDI ToF-ToF.
Our research utilises a range of MS-based techniques including - top-down and middle down fragmentation, ion-mobility mass spectrometry, native mass spectrometry, and hydrogen/deuterium exchange. In addition, MS-based studies are supported by complementary biochemical analysis and structural biology approaches.
Websites
www.clarkelab.co.uk
Qualifications
MChem, PhD
Fingerprint
Dive into the research topics where David Clarke is active. These topic labels come from the works of this person. Together they form a unique fingerprint.
- 1 Similar Profiles
Network
Recent external collaboration on country/territory level. Dive into details by clicking on the dots or
-
Single-Molecule Two-Color Coincidence Detection of Unlabeled alpha-Synuclein Aggregates
Chappard, A., Leighton, C., Saleeb, R. S., Jeacock, K., Ball, S., Morris, K., Kantelberg, O., Lee, J. E., Zacco, E., Pastore, A., Sunde, M., Clarke, D. J., Downey, P., Kunath, T. & Horrocks, M. H., 3 Apr 2023, In: Angewandte Chemie - International Edition. 62, 15, e202216771.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Determining the location of the α-synuclein dimer Interface using native top-down fragmentation and isotope depletion-mass spectrometry
Jeacock, K., Chappard, A., Gallagher, K. J., Mackay, C. L., Kilgour, D. P. A., Horrocks, M. H., Kunath, T. & Clarke, D. J., 28 Mar 2023, In: Journal of the American Society for Mass Spectrometry. 10 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics
Tsiamis, A., Buchoux, A., Mahon, S., Walton, A. J., Smith, S., Clarke, D. J. & Stokes, A. A., 25 Feb 2023, (E-pub ahead of print) In: Micromachines. 14, 3, 537.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
A mechanistic evaluation of human beta defensin 2 mediated protection of human skin barrier in vitro
Shelley, J. R., Mchugh, B. J., Wills, J., Dorin, J. R., Weller, R., Clarke, D. J. & Davidson, D. J., 8 Feb 2023, In: Scientific Reports. 13, 1, 2271.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Pathological structural conversion of α-synuclein at the mitochondria induces neuronal toxicity
Choi, M. L., Chappard, A., Singh, B. P., Maclachlan, C., Rodrigues, M., Fedotova, E. I., Berezhnov, A. V., De, S., Peddie, C. J., Athauda, D., Virdi, G. S., Zhang, W., Evans, J. R., Wernick, A. I., Zanjani, Z. S., Angelova, P. R., Esteras, N., Vinokurov, A. Y., Morris, K., Jeacock, K., & 10 others, Sep 2022, In: Nature Neuroscience. 25, 9, p. 1134-1148Research output: Contribution to journal › Article › peer-review
Open AccessFile
Activities
-
Invited Talk
David Clarke (Assessor)
12 Feb 2019 → 14 Feb 2019Activity: Academic talk or presentation types › Oral presentation
-
Analytical Chemistry (Journal)
David Clarke (Peer reviewer)
14 Mar 2019Activity: Publication peer-review and editorial work types › Publication peer-review
-
Chemical Science (Journal)
David Clarke (Peer reviewer)
2 Apr 2019Activity: Publication peer-review and editorial work types › Publication peer-review
-
Analyst (Journal)
David Clarke (Peer reviewer)
6 Aug 2018Activity: Publication peer-review and editorial work types › Publication peer-review
-
Inspiring Science: Science Fest
Eleanor Campbell (Organiser), Anna-Maria Maciejuk (Contributor), Colin Campbell (Invited speaker) & David Clarke (Invited speaker)
12 Jan 2018 → 13 Jan 2018Activity: Participating in or organising an event types › Public Engagement – Festival/Exhibition
Projects
- 11 Finished
-
-
Native Mass Spectrometry analysis of the P2X7 membrane protein complex
Menzies, R. & Clarke, D.
1/01/17 → 31/12/17
Project: University Awarded Project Funding
-
Engineering a novel industrial bio-transformation pathway for surfactant manufacture
1/10/16 → 1/11/17
Project: Research
-
Datasets
-
Native MS Analysis of Encapsulated Ferritin from Rhodospirillum rubrum
Clarke, D. (Creator), University of Edinburgh, 22 Jul 2016
DOI: 10.7488/ds/1449
Dataset
-
Isotope Depletion Mass Spectrometry (ID MS) for Enhanced Top-Down Protein Fragmentation - Datasets
Palasser, M. (Creator), Gallagher, K. (Creator) & Clarke, D. (Creator), Edinburgh DataShare, 27 Sep 2018
DOI: 10.7488/ds/2446
Dataset
-
Top-Down Protein Electron Capture Dissociation Mass Spectrometry Datasets
Clarke, D. (Creator), University of Edinburgh, 2016
DOI: 10.7488/ds/1448
Dataset
-
Optimization of sprayer for matrix application in MALDI mass spectrometry imaging
Tucker, L. (Creator) & Clarke, D. (Depositor), Edinburgh DataShare, 6 Feb 2018
DOI: 10.7488/ds/2300
Dataset
-
Determining the Location of the Alpha-Synuclein Dimer Interface using Native Top-Down Fragmentation and Isotope Depletion-Mass Spectrometry
Clarke, D. (Creator) & Jeacock, K. (Creator), Edinburgh DataShare, 20 Feb 2023
DOI: https://doi.org/10.7488/ds/3811
Dataset