Research output per year
Research output per year
Personal profile
Current Research Interests
Biomolecular Mass Spectrometry, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), Top-Down Mass Spectrometry, Protein Chemistry, Enzymology, Structural Biology.
Biography
The core research theme of our group is the application of modern techniques in mass spectrometry (MS) for the functional and structural characterisation of biological systems. The School of Chemistry houses an excellent array of MS instrumentation - including FT-ICR, an ion mobility equipped Q-Tof, an extended mass range ESI Q-tof, and a MALDI ToF-ToF.
Our research utilises a range of MS-based techniques including - top-down and middle down fragmentation, ion-mobility mass spectrometry, native mass spectrometry, and hydrogen/deuterium exchange. In addition, MS-based studies are supported by complementary biochemical analysis and structural biology approaches.
Websites
www.clarkelab.co.uk
Qualifications
MChem, PhD
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Research output
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Dissecting the structural and functional roles of a putative metal entry site in encapsulated ferritins
Piergentili, C., Ross, J., He, D., Gallagher, K. J., Stanley, W. A., Adam, L., Mackay, C. L., Baslé, A., Waldron, K. J., Clarke, D. J. & Marles-Wright, J., 2 Sep 2020, In: Journal of Biological Chemistry.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Isotope Depletion Mass Spectrometry (ID-MS) for Accurate Mass Determination and Improved Top-Down Sequence Coverage of Intact Proteins
Gallagher, K. J., Palasser, M., Hughes, S., Mackay, C. L., Kilgour, D. P. A. & Clarke, D. J., 31 Jan 2020, In: Journal of the American Society for Mass Spectrometry.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Mass spectrometry reveals the assembly pathway of encapsulated ferritins and highlights a dynamic ferroxidase interface
Ross, J., Lambert, T., Piergentili, C., He, D., Waldron, K. J., Mackay, C. L., Marles-wright, J. & Clarke, D. J., 13 Feb 2020, In: Chemical Communications.Research output: Contribution to journal › Article › peer-review
Open AccessFile -
Conservation of the structural and functional architecture of encapsulated ferritins in bacteria and archaea
He, D., Piergentili, C., Ross, J., Tarrant, E., Tuck, L. R., Mackay, C. L., Mciver, Z., Waldron, K. J., Clarke, D. J. & Marles-wright, J., 5 Mar 2019, In: Biochemical Journal. 476, 6, p. 975-989Research output: Contribution to journal › Article › peer-review
Open AccessFile -
High resolution fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) for the characterisation of enzymatic processing of commercial lignin
Echavarri-Bravo, V., Tinzl, M., Kew, W., Cruickshank, F., Logan Mackay, C., Clarke, D. J. & Horsfall, L. E., 25 Sep 2019, In: New Biotechnology. 52, p. 1-8 8 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile
Activities
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Invited Talk
David Clarke (Assessor)
12 Feb 2019 → 14 Feb 2019Activity: Academic talk or presentation types › Oral presentation
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Chemical Science (Journal)
David Clarke (Peer reviewer)
2 Apr 2019Activity: Publication peer-review and editorial work types › Publication peer-review
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Analytical Chemistry (Journal)
David Clarke (Peer reviewer)
14 Mar 2019Activity: Publication peer-review and editorial work types › Publication peer-review
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Isotope Depletion Mass Spectrometry for Improved Top-Down Fragmentation
David Clarke (Assessor)
17 Jul 2018Activity: Academic talk or presentation types › Invited talk
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Analyst (Journal)
David Clarke (Peer reviewer)
6 Aug 2018Activity: Publication peer-review and editorial work types › Publication peer-review
Projects
- 11 Finished
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Native Mass Spectrometry analysis of the P2X7 membrane protein complex
Menzies, R. & Clarke, D.
1/01/17 → 31/12/17
Project: University Awarded Project Funding
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Engineering a novel industrial bio-transformation pathway for surfactant manufacture
1/10/16 → 1/11/17
Project: Research
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Inhibiting the HOP-Hsp90 interaction through fragment based drug discovery
1/09/16 → 30/09/19
Project: Research
Datasets
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Native MS Analysis of Encapsulated Ferritin from Rhodospirillum rubrum
Clarke, D. (Creator), University of Edinburgh, 22 Jul 2016
DOI: 10.7488/ds/1449
Dataset
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Mass Spectrometry Analysis of Encapsulated Ferritins from Haliangium ochraceum – Native IM-MS and HDX-MS
Ross, J. (Creator), Lambert, T. (Creator), He, D. (Creator), Piergentili, C. (Creator), Tarrant, E. (Creator), Waldron, K. (Creator), Marles-Wright, J. (Creator), Clarke, D. (Creator) & Mackay, L. (Creator), Edinburgh DataShare, 7 Oct 2019
DOI: 10.7488/ds/2630, https://doi.org/10.26434/chemrxiv.9901346.v1
Dataset
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Optimization of sprayer for matrix application in MALDI mass spectrometry imaging
Tucker, L. (Creator) & Clarke, D. (Depositor), Edinburgh DataShare, 6 Feb 2018
DOI: 10.7488/ds/2300
Dataset
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Use of Isotopically Labelled Substrates Reveals Kinetic Differences Between Human and Bacterial Serine Palmitoyltransferase
Harrison, P. (Creator), Clarke, D. (Creator), Kelly, V. (Creator) & Campopiano, D. (Creator), Edinburgh DataShare, 4 Mar 2019
DOI: 10.7488/ds/2503, http://www.jlr.org/content/early/2019/02/21/jlr.M089367.long
Dataset
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ESI-(-)-FT-ICR MS of 85 Scotch Whisky Samples
Kew, W. (Creator), Uhrin, D. (Creator), Clarke, D. (Creator) & Goodall, I. (Creator), Edinburgh DataShare, 18 Oct 2016
DOI: 10.7488/ds/1486
Dataset