Kevin Hardwick

2024

Conserved signalling functions for Mps1, Mad1 and Mad2 in the Cryptococcus neoformans spindle checkpoint

Aktar, K., Davies, T., Leontiou, I., Clark, I., Spanos, C., Wallace, E., Tuck, L., Jeyaprakash, A. A. & Hardwick, K. G., 3 Jun 2024, In: PLoS Genetics. 20, 6, p. e1011302 29 p., e1011302.

Research output: Contribution to journal › Article › peer-review

Open Access

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2023

Cell-cycle control: Timing is everything for the Plk1–Bub1 partnership

Davies, T. & Hardwick, K. G., 5 Jun 2023, In: Current Biology. 33, 11, p. R447-R449

Research output: Contribution to journal › Comment/debate › peer-review

mps1 and mad mutations reduce Cryptococcus neoformans titan cell viability

Aktar, K., Davies, T., Leontiou, I., Clark, I. B. N., Spanos, C., Wallace, E., Tuck, L., Jeyaprakash, A. A. & Hardwick, K. G., 9 Apr 2023, bioRxiv, 46 p.

Research output: Working paper › Preprint

Open Access

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2022

Bub1 kinase acts as a signalling hub for the entire Cryptococcus neoformans spindle assembly checkpoint pathway

Leontiou, I., Davies, T., Clark, I., Aktar, K., Suresh, A. P., Abad, M. A., Spanos, C., Lee, K-T., Bahn, Y-S., Jeyaprakash, A. A. & Hardwick, K. G., 22 Sept 2022, (bioRxiv).

Research output: Working paper › Preprint

2019

The Bub1-TPR domain interacts directly with Mad3 to generate robust spindle checkpoint arrest

Leontiou, I., London, N., May, K. M., Ma, Y., Grzesiak, L., Medina-Pritchard, B., Amin, P., Jeyaprakash, A. A., Biggins, S. & Hardwick, K. G., 22 Jul 2019, In: Current biology : CB. 29, 14

Research output: Contribution to journal › Article › peer-review

Open Access

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2018

Regulated reconstitution of spindle checkpoint arrest and silencing through chemically induced dimerisation, in vivo

Amin, P., Chafraidh, S. S. N., Leontiou, I. & Hardwick, K. G., 4 Oct 2018, In: Journal of Cell Science. 132, 4, jcs.219766.

Research output: Contribution to journal › Article › peer-review

Open Access

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2017

Fission yeast Apc15 stabilises MCC-Cdc20-APC/C complexes ensuring efficient Cdc20 ubiquitination and checkpoint arrest

May, K. M., Paldi, F. & Hardwick, K. G., 24 Apr 2017, In: Current Biology. 27, 8, p. 1221-1228 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast

Meadows, J. C., Lancaster, T. C., Buttrick, G. J., Sochaj, A. M., Messin, L. J., Mora-Santos, M. D. M., Hardwick, K. & Millar, J. B. A., 7 Feb 2017, In: Cell Reports. 18, 6, p. 1422-1433 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Generation of a spindle checkpoint arrest from synthetic signaling assemblies

Yuan, I., Leontiou, I., Amin, P., May, K., Soper ní chafraidh, S., Zlámalová, E. & Hardwick, K., 9 Jan 2017, In: Current Biology. 27, 1, p. 137-143 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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2016

Mps1Mph1 kinase phosphorylates Mad3 to inhibit Cdc20Slp1-APC/C and maintain spindle checkpoint arrests

Hardwick, K., Rappsilber, J., Van Der Sar, S., Syred, H. M., Sen, O., Paraskevopoulos, K., May, K., Zich, J., Patel, H., Moresco, J. J., Sarkeshik, A. & Yates III, J. R., 16 Feb 2016, In: PLoS Genetics. 12, 2, e1005834.

Research output: Contribution to journal › Article › peer-review

Open Access

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2015

The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast

Folco, H. D., Campbell, C. S., May, K. M., Espinoza, C. A., Oegema, K., Hardwick, K. G., Grewal, S. I. S. & Desai, A., 1 Jan 2015, In: Current Biology. 25, 3, p. 348-356 9 p.

Research output: Contribution to journal › Article › peer-review

2014

Dss1 Is a 26S Proteasome Ubiquitin Receptor

Paraskevopoulos, K., Kriegenburg, F., Tatham, M. H., Rösner, H. I., Medina, B., Larsen, I. B., Brandstrup, R., Hardwick, K. G., Hay, R. T., Kragelund, B. B., Hartmann-Petersen, R. & Gordon, C., 6 Nov 2014, In: Molecular Cell. 56, 3, p. 453-61 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Identification of a BET Family Bromodomain/Casein Kinase II/TAF-Containing Complex as a Regulator of Mitotic Condensin Function

Kim, H-S., Mukhopadhyay, R., Rothbart, S. B., Silva, A. C., Vanoosthuyse, V., Radovani, E., Kislinger, T., Roguev, A., Ryan, C. J., Xu, J., Jahari, H., Hardwick, K. G., Greenblatt, J. F., Krogan, N. J., Fillingham, J. S., Strahl, B. D., Bouhassira, E. E., Edelmann, W. & Keogh, M-C., 13 Mar 2014, In: Cell Reports. 6, 5, p. 892-905 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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CPF-Associated Phosphatase Activity Opposes Condensin-Mediated Chromosome Condensation

Vanoosthuyse, V., Legros, P., van der Sar, S. J. A., Yvert, G., Toda, K., Le Bihan, T., Watanabe, Y., Hardwick, K. & Bernard, P., 1 Jan 2014, In: PLoS Genetics. 10, 6, e1004415.

Research output: Contribution to journal › Article › peer-review

Open Access

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2013

Kindlin-1 regulates mitotic spindle formation by interacting with integrins and Plk-1

Patel, H., Zich, J., Serrels, B., Rickman, C., Hardwick, K. G., Frame, M. C. & Brunton, V. G., Jun 2013, In: Nature Communications. 4, n/a, 2056.

Research output: Contribution to journal › Article › peer-review

2012

Kinase Activity of Fission Yeast Mph1 Is Required for Mad2 and Mad3 to Stably Bind the Anaphase Promoting Complex

Zich, J., Sochaj, A. M., Syred, H. M., Milne, L., Cook, A. G., Ohkura, H., Rappsilber, J. & Hardwick, K. G., 21 Feb 2012, In: Current Biology. 22, 4, p. 296–301

Research output: Contribution to journal › Article › peer-review

Open Access

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Phosphodependent Recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 Kinase Maintains the Spindle Checkpoint

Shepperd, L. A., Meadows, J. C., Sochaj, A. M., Lancaster, T. C., Zou, J., Buttrick, G. J., Rappsilber, J., Hardwick, K. G. & Millar, J. B. A., 2012, In: Current Biology. 22, 10, p. 891-899 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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2011

Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B

Buttrick, G. J., Meadows, J. C., Lancaster, T. C., Vanoosthuyse, V., Shepperd, L. A., Hoe, K-L., Kim, D-U., Park, H-O., Hardwick, K. G. & Millar, J. B. A., 2011, In: Molecular Biology of the Cell. 22, 23, p. 4486-502 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors

Meadows, J. C., Shepperd, L. A., Vanoosthuyse, V., Lancaster, T. C., Sochaj, A. M., Buttrick, G. J., Hardwick, K. G. & Millar, J. B. A., 2011, In: Developmental Cell. 20, 6, p. 739-750 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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2010

Getting down to the phosphorylated 'nuts and bolts' of spindle checkpoint signalling

Zich, J. & Hardwick, K. G., 1 Jan 2010, In: Trends in biochemical sciences. 35, 1, p. 18-27 10 p.

Research output: Contribution to journal › Article › peer-review

Spindle checkpoint silencing: ensuring rapid and concerted anaphase onset

Hardwick, K. G. & Shah, J. V., 2010, In: F1000 biology reports. 2, p. 55

Research output: Contribution to journal › Article › peer-review

Open Access

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2009

Overcoming inhibition in the spindle checkpoint

Vanoosthuyse, V. & Hardwick, K. G., Dec 2009, In: Genes & Development. 23, 24, p. 2799-805 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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A Novel Protein Phosphatase 1-Dependent Spindle Checkpoint Silencing Mechanism

Vanoosthuyse, V. & Hardwick, K. G., Jul 2009, In: Current Biology. 19, 14, p. 1176-1181 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe

Kim, H-S., Vanoosthuyse, V., Fillingham, J., Roguev, A., Watt, S., Kislinger, T., Treyer, A., Carpenter, L. R., Bennett, C. S., Emili, A., Greenblatt, J. F., Hardwick, K. G., Krogan, N. J., Bähler, J. & Keogh, M-C., 2009, In: Nature Structural & Molecular Biology. 16, 12, p. 1286-93 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Bub3p facilitates spindle checkpoint silencing in fission yeast

Vanoosthuyse, V., Meadows, J. C., van der Sar, S. J. A., Millar, J. B. A. & Hardwick, K. G., 2009, In: Molecular Biology of the Cell. 20, 24, p. 5096-105 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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The spindle checkpoint: Assays for the analysis of spindle checkpoint arrest and recovery

Fernius, J. & Hardwick, K. G., 2009, In: Methods in Molecular Biology. 545, p. 243-58 16 p.

Research output: Contribution to journal › Article › peer-review

2008

The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C)

Sczaniecka, M., Feoktistova, A., May, K. M., Chen, J-S., Blyth, J., Gould, K. L. & Hardwick, K. G., 22 Aug 2008, In: Journal of Biological Chemistry. 283, 34, p. 23039-23047 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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The spindle checkpoint: how do cells delay anaphase onset?

Sczaniecka, M. M. & Hardwick, K. G., 2008, In: SEB experimental biology series. 59, p. 243-56 14 p.

Research output: Contribution to journal › Article

2007

Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes

Rischitor, P. E., May, K. M. & Hardwick, K. G., 2007, In: PLoS ONE. 2, 12, 6 p., e1342.

Research output: Contribution to journal › Article › peer-review

Open Access

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Bub1 kinase targets Sgo1 to ensure efficient chromosome biorientation in budding yeast mitosis

Fernius, J. & Hardwick, K. G., 2007, In: PLoS Genetics. 3, 11, 14 p., e213.

Research output: Contribution to journal › Article › peer-review

Open Access

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Comment on "A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint"

Taylor, S. S., Hardwick, K. G., Sawin, K. E., Biggins, S., Piatti, S., Khodjakov, A., Rieder, C. L., Salmon, E. D. & Musacchio, A., 2007, In: Science. 316, 5827, p. 982 1 p.

Research output: Contribution to journal › Comment/debate › peer-review

Open Access

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Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores

King, E. M. J., Rachidi, N., Morrice, N., Hardwick, K. G. & Stark, M. J. R., 2007, In: Genes & Development. 21, 10, p. 1163-8 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint

King, E. M. J., van der Sar, S. J. A. & Hardwick, K. G., 2007, In: PLoS ONE. 2, 4, 12 p., e342.

Research output: Contribution to journal › Article › peer-review

Open Access

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Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function

Anderson, V. E., Prudden, J., Prochnik, S., Giddings, T. H. & Hardwick, K. G., 2007, In: Molecular Biology of the Cell. 18, 6, p. 2047-56 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis

Vanoosthuyse, V., Prykhozhij, S. & Hardwick, K. G., 2007, In: Molecular Biology of the Cell. 18, 5, p. 1657-69 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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2006

The spindle checkpoint

May, K. M. & Hardwick, K. G., 2006, In: Journal of Cell Science. 119, Pt 20, p. 4139-42 4 p.

Research output: Contribution to journal › Article › peer-review

2005

The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function

Kadura, S., He, X., Vanoosthuyse, V., Hardwick, K. G. & Sazer, S., 2005, In: Molecular Biology of the Cell. 16, 1, p. 385-95 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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2004

Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p

Vanoosthuyse, V., Valsdottir, R., Javerzat, J-P. & Hardwick, K. G., 2004, In: Molecular and Cellular Biology. 24, 22, p. 9786-801 16 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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2003

Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts

Campbell, L. & Hardwick, K. G., 2003, In: Journal of Cell Science. 116, Pt 4, p. 617-28 12 p.

Research output: Contribution to journal › Article › peer-review

The complexity of Bub1 regulation--phosphorylation, phosphorylation, phosphorylation

Vanoosthuyse, V. & Hardwick, K. G., 2003, In: Cell Cycle. 2, 2, p. 118-9 2 p.

Research output: Contribution to journal › Article

2002

The spindle checkpoint: Structural insights into dynamic signalling

Musacchio, A. & Hardwick, K. G., 1 Oct 2002, In: Nature reviews Molecular cell biology. 3, 10, p. 731-741 11 p.

Research output: Contribution to journal › Article › peer-review

Distinct chromosome segregation roles for spindle checkpoint proteins

Warren, C. D., Brady, D. M., Johnston, R. C., Hanna, J. S., Hardwick, K. G. & Spencer, F. A., 2002, In: Molecular Biology of the Cell. 13, 9, p. 3029-41 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Fission Yeast Mad3p Is Required for Mad2p To Inhibit the Anaphase-Promoting Complex and Localizes to Kinetochores in a Bub1p-, Bub3p-, and Mph1p-Dependent Manner

Millband, D. N. & Hardwick, K. G., 2002, In: Molecular and Cellular Biology. 22, 8, p. 2728-42 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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The awesome power of multiple model systems: interpreting the complex nature of spindle checkpoint signaling

Millband, D. N., Campbell, L. & Hardwick, K. G., 2002, In: Trends In Cell Biology. 12, 5, p. 205-9 5 p.

Research output: Contribution to journal › Article › peer-review

2000

Cdc28 activates exit from mitosis in budding yeast

Rudner, A. D., Hardwick, K. G. & Murray, A. W., 2000, In: Journal of Cell Biology. 149, 7, p. 1361-76 16 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function

Brady, D. M. & Hardwick, K. G., 2000, In: Current biology : CB. 10, 11, p. 675-8 4 p.

Research output: Contribution to journal › Article › peer-review

MAD3 Encodes a Novel Component of the Spindle Checkpoint Which Interacts with Bub3p, Cdc20p, and Mad2p

Hardwick, K. G., Johnston, R. C., Smith, D. L. & Murray, A. W., 2000, In: Journal of Cell Biology. 148, 5, p. 871-82 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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Kevin Hardwick

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