Pat Langridge-Smith

Search results

• 2016

  Mass spectrometry analysis of the oxidation states of the pro-oncogenic protein anterior gradient-2 reveals covalent dimerization via an intermolecular disulphide bond

  Clarke, D. J., Murray, E., Faktor, J., Mohtar, A., Vojtesek, B., MacKay, C. L., Smith, P. L. & Hupp, T. R., May 2016, In: BBA - Bioenergetics.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Dimerization 100%
  • Disulfides 93%
  • Cysteine 83%
  • Mass Spectrometry 79%
  • Disulfide 72%

• Determination of Protein Thiol Reduction Potential by Isotope Labeling and Intact Mass Measurement

  Thurlow, S. E., Kilgour, D. P., Campopiano, D. J., Mackay, C. L., Langridge-Smith, P. R. R., Clarke, D. J. & Campbell, C. J., 1 Mar 2016, In: Analytical Chemistry. 88, 5, p. 2727-33 7 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Reduction Potential 100%
  • Isotope 83%
  • Thiol 81%
  • Protein 39%
  • Thioredoxin 30%

• Screen-printed digital microfluidics combined with surface acoustic wave nebulization for hydrogen-deuterium exchange measurements

  Monkkonen, L., Edgar, J. S., Winters, D., Heron, S. R., Mackay, C. L., Masselon, C. D., Stokes, A. A., Langridge-Smith, P. R. R. & Goodlett, D. R., Mar 2016, In: Journal of Chromatography A. 1439, p. 161-6 6 p.

  Research output: Contribution to journal › Article › peer-review

  File

  • Deuterium Exchange Measurement 100%
  • Surface Acoustic Wave 64%
  • Deuterium 63%
  • Microfluidics 54%
  • Hydrogen 42%
• 2015

  Nebulization of water/glycerol droplets generated by ZnO/Si surface acoustic wave devices

  Guo, Y. J., Denison, A. P., Li, Y., Luo, J., Zhu, X. T., Mackay, C. L. M., Langridge-Smith, P., Walton, A. & Fu, R., 2015, In: Microfluidics and Nanofluidics. 19, p. 273-282 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Acoustic surface wave devices 100%
  • surface acoustic wave devices 82%
  • Glycerol 79%
  • glycerols 74%
  • Surface Acoustic Wave 68%
• 2014

  Dissecting the Dynamic Conformations of the Metamorphic Protein Lymphotactin

  Harvey, S. R., Porrini, M., Konijnenberg, A., Clarke, D. J., Tyler, R. C., Langridge-Smith, P. R. R., MacPhee, C. E., Volkman, B. F. & Barran, P. E., 30 Oct 2014, In: Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry). 118, 43, p. 12348-59 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Conformations 100%
  • Dimers 95%
  • Monomers 74%
  • Proteins 72%
  • fragmentation 71%

• A novel two-laser interface for coupling capillary electrochromatography with ion-trap time-of-flight mass spectrometry

  Simpson, D. C., Yates, A. J., Knox, J. H. & Langridge-smith, P. R. R., 15 Apr 2014, In: International Journal of Mass Spectrometry. 363, p. 8-15

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Mass spectrometry 100%
  • Ion Trap 85%
  • Time-of-Flight Mass Spectrometry 78%
  • mass spectroscopy 75%
  • Ions 71%
• 2013

  Mass Spectrometry Imaging for Dissecting Steroid Intracrinology within Target Tissues

  Cobice, D. F., Mackay, C. L., Goodwin, R. J. A., McBride, A., Langridge-Smith, P. R., Webster, S. P., Walker, B. R. & Andrew, R., 3 Dec 2013, In: Analytical Chemistry. 85, 23, p. 11576-11584 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Imaging Mass Spectrometry 100%
  • Steroid 70%
  • 11-Dehydrocorticosterone 55%
  • Corticosterone 42%
  • 7-Ketocholesterol 25%

• The chemical basis of serine palmitoyltransferase inhibition by myriocin

  Wadsworth, J. M., Clarke, D. J., McMahon, S. A., Lowther, J. P., Beattie, A. E., Langridge-Smith, P. R. R., Broughton, H. B., Dunn, T. M., Naismith, J. H. & Campopiano, D. J., 25 Sep 2013, In: Journal of the American Chemical Society. 135, 38, p. 14276-85 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Myriocin 100%
  • thermozymocidin 94%
  • Serine C-Palmitoyltransferase 92%
  • Enzymes 62%
  • Phosphates 62%

• Competition between glutathione and DNA oligonucleotides for ruthenium(II) arene anticancer complexes

  Wang, F., Xu, J., Wu, K., Weidt, S. K., Mackay, C. L., Langridge-Smith, P. R. R. & Sadler, P. J., 7 Mar 2013, In: Dalton Transactions. 42, 9, p. 3188-3195 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Glutathione 100%
  • Oligonucleotide 98%
  • Arene 88%
  • Adduct 47%
  • Guanine 43%

• Redox regulation of tumour suppressor protein p53: identification of the sites of hydrogen peroxide oxidation and glutathionylation

  Scotcher, J., Clarke, D. J., Mackay, C. L., Hupp, T., Sadler, P. J. & Langridge-Smith, P. R. R., Mar 2013, In: Chemical Science. 4, 3, p. 1257-1269 13 p.

  Research output: Contribution to journal › Article › peer-review

  • Tumor Suppressor Protein 100%
  • Hydrogen Peroxide 48%
  • Oxidation Reaction 28%
  • Cysteine 21%
  • Disulfide 20%
• 2012

  Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation

  Goodwin, R. J. A., Nilsson, A., Borg, D., Langridge-Smith, P. R. R., Harrison, D. J., Mackay, C. L., Iverson, S. L. & Andrén, P. E., 30 Aug 2012, In: Journal of proteomics. 75, 16, p. 4912-4920 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry 100%
  • Tiotropium 83%
  • Carbon 82%
  • Hot Temperature 64%
  • Adhesive Tape 61%

• Test Structures for Characterizing the Integration of EWOD and SAW Technologies for Microfluidics

  Li, Y., Fu, R. Y., Winters, D., Flynn, B. W., Parkes, B., Brodie, D. S., Liu, Y., Terry, J., Haworth, L. I., Bunting, A. S., Stevenson, T., Smith, S., MacKay, C. L., Langridge-Smith, P. R. R., Stokes, A. A. & Walton, A. J., 3 Aug 2012, In: IEEE Transactions on Semiconductor Manufacturing. 25, 3, p. 323-330 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Surface Acoustic Wave 100%
  • Surface waves 79%
  • Microfluidics 75%
  • Wetting 72%
  • wetting 67%

• L-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5 '-phosphate-thiazolidine adduct

  Lowther, J., Beattie, A. E., Langridge-Smith, P. R. R., Clarke, D. J. & Campopiano, D. J., Aug 2012, In: Medchemcomm. 3, 8, p. 1003-1008 6 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • L-Penicillamine 100%
  • Thiazolidines 99%
  • Serine C-Palmitoyltransferase 97%
  • Pyridoxal Phosphate 76%
  • Penicillamine 73%

• Lactate, a product of glycolytic metabolism, inhibits histone deacetylase activity and promotes changes in gene expression

  Latham, T., Mackay, L., Sproul, D., Karim, M., Culley, J., Harrison, D. J., Hayward, L., Langridge-Smith, P., Gilbert, N. & Ramsahoye, B. H., Jun 2012, In: Nucleic Acids Research. 40, 11, p. 4794-4803 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Histone Deacetylases 100%
  • Lactic Acid 76%
  • Gene Expression 51%
  • Genes 34%
  • Histone Deacetylase Inhibitors 33%

• An affinity purification procedure to isolate oxidized p53

  Scotcher, J., Clarke, D. J. & Langridge-Smith, P. R. R., 1 Jan 2012, In: Analytical Biochemistry. 420, 1, p. 96-98 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Biotin 100%
  • Purification 92%
  • Cysteine 85%
  • Tumor Suppressor Protein 71%
  • Tumor Suppressor Protein p53 60%

• Appropriate degree of trust: deriving confidence metrics for automatic peak assignment in high-resolution mass spectrometry

  Kilgour, D. P. A., Mackay, C. L., Langridge-Smith, P. R. R. & O'Connor, P. B., 2012, In: Analytical Chemistry. 84, 17, p. 7431-5 5 p.

  Research output: Contribution to journal › Article › peer-review

  • High Resolution Mass Spectrometry (HRMS) 100%
  • Chemical Element 70%
  • Error 68%
  • Ion 36%

• Ferric ion (hydr)oxo clusters in the "Venus flytrap" cleft of FbpA: Mössbauer, calorimetric and mass spectrometric studies

  Mukherjee, A., Bilton, P. R., Mackay, L., Janoschka, A., Zhu, H., Rea, D., Langridge-Smith, P. R. R., Campopiano, D. J., Teschner, T., Trautwein, A. X., Schünemann, V. & Sadler, P. J., 2012, In: JBIC Journal of Biological Inorganic Chemistry.

  Research output: Contribution to journal › Article › peer-review

  • Droseraceae 100%
  • Iron 44%
  • Ions 44%
  • Molecular Cluster 34%
  • Ion 20%

• Use of an artificial immune system derived method for the charge state assignment of small-molecule mass spectra

  Kilgour, D. P. A., Mackay, C. L., Langridge-Smith, P. R. R. & O'Connor, P. B., 2012, In: Analytical Chemistry. 84, 17, p. 7436-9 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Mass Spectrum 100%
  • Ion 45%
• 2011

  Identification of Two Reactive Cysteine Residues in the Tumor Suppressor Protein p53 Using Top-Down FTICR Mass Spectrometry

  Scotcher, J., Clarke, D., Weidt, S., Mackay, L., Hupp, T., Sadler, P. & Langridge-Smith, P., May 2011, In: Journal of the American Society for Mass Spectrometry. 22, 5, p. 888-897

  Research output: Contribution to journal › Article › peer-review

  • Tumor Suppressor Protein 100%
  • Tumor Suppressor Protein p53 84%
  • Cysteine Residue 66%
  • Cysteine 59%
  • Mass Spectrometry 57%

• Mapping a noncovalent protein-peptide interface by top-down FTICR mass spectrometry using electron capture dissociation

  Clarke, D. J., Murray, E., Hupp, T., Mackay, C. L. & Langridge-Smith, P. R. R., 2011, In: Journal of the American Society for Mass Spectrometry. 22, 8, p. 1432-40 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Electron-Capture Dissociation 100%
  • Mass Spectrometry 62%
  • Electrons 55%
  • Peptides 44%
  • Fragmentation 34%

• Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

  Goodwin, R. J. A., Pitt, A. R., Harrison, D., Weidt, S. K., Langridge-Smith, P. R. R., Barrett, M. P. & Logan Mackay, C., 2011, In: Rapid communications in mass spectrometry. 25, 7, p. 969-72 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Imaging Mass Spectrometry 100%
  • Laser Desorption 87%
  • FT/ICR Mass Spectrometry 87%
  • Ionization 60%
  • Spreading 40%
• 2010

  Online Quench-Flow Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Elucidating Kinetic and Chemical Enzymatic Reaction Mechanisms

  Clarke, D. J., Stokes, A., Langridge-Smith, P. & Mackay, C. L., 1 Mar 2010, In: Analytical Chemistry. 82, 5, p. 1897-1904 8 p.

  Research output: Contribution to journal › Article › peer-review

  • FT/ICR Mass Spectrometry 100%
  • Enzymatic Reaction 87%
  • Electrospray Ionization 80%
  • Fragmentation 36%
  • Electron-Capture Dissociation 31%

• Top-down protein sequencing by CID and ECD using desorption electrospray ionisation (DESI) and high-field FTICR mass spectrometry

  Stokes, A. A., Clarke, D. J., Weidt, S., Langridge-Smith, P. & Mackay, C. L., 1 Jan 2010, In: International Journal of Mass Spectrometry. 289, 1, p. 54-57 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Electrospray ionization 100%
  • Electron-Capture Dissociation 75%
  • sequencing 71%
  • Mass spectrometry 66%
  • Desorption 59%

• Subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins based on catalytic activity

  Clarke, D. J., Ortega, X. P., Mackay, C. L., Valvano, M. A., Govan, J. R. W., Campopiano, D. J., Langridge-Smith, P. & Brown, A. R., 2010, In: Biochemistry. 49, 6, p. 1319-30 12 p.

  Research output: Contribution to journal › Article › peer-review

  • bacterioferritin 100%
  • Burkholderia cenocepacia 92%
  • Peroxiredoxins 76%
  • Bacterial Proteins 68%
  • Cysteine 46%
• 2009

  Interrogating the molecular details of the peroxiredoxin activity of the Escherichia coli bacterioferritin comigratory protein using high-resolution mass spectrometry

  Clarke, D. J., Mackay, C. L., Campopiano, D. J., Langridge-Smith, P. & Brown, A. R., May 2009, In: Biochemistry. 48, 18, p. 3904-14 11 p.

  Research output: Contribution to journal › Article › peer-review

  • bacterioferritin 100%
  • Peroxiredoxins 76%
  • High Resolution Mass Spectrometry (HRMS) 60%
  • Mass Spectrometry 50%
  • Escherichia coli 39%

• Precursor ion independent algorithm for top-down shotgun proteomics

  Tsai, Y., Scherl, A., Shaw, J., Mackay, L., Schaffer, S., Langridge-Smith, P. & Goodlett, D., 2009, In: Journal of the American Society for Mass Spectrometry. 20, 11, p. 2154-2166

  Research output: Contribution to journal › Article › peer-review

  • Firearms 100%
  • Proteomics 80%
  • Ions 72%
  • Tandem Mass Spectrum 59%
  • Protein Precursors 36%
• 2008

  Sensitive, Specific and Quantitative FT-ICR Mass Spectrometry of Combinatorial Post-Translational Modifications in Intact Histone H4

  Mackay, L., Ramsahoye, B., Burgess, K., Cook, K., Weidt, S., Creanor, J., Harrison, D., Langridge-Smith, P., Hupp, T. & Hayward, L., Jun 2008, In: Analytical Chemistry. 80, 11, p. 4147-4153

  Research output: Contribution to journal › Article › peer-review

  • FT/ICR Mass Spectrometry 100%
  • Histone 93%
  • Modification 48%
  • Electron-Capture Dissociation 20%
  • Acetylation 14%

• Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass spectrometry combined with (18)O-labeling

  Wang, F., Weidt, S., Xu, J., Mackay, C. L., Langridge-Smith, P. R. R. & Sadler, P. J., 2008, In: Journal of the American Society for Mass Spectrometry. 19, 4, p. 544-549 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Ruthenium 100%
  • Cyclotrons 95%
  • Fourier Analysis 74%
  • Liquid Chromatography 65%
  • Glutathione 57%

• Sensitive, specific, and quantitative FTICR mass spectrometry of combinatorial post-translational modifications in intact histone H4

  Mackay, C. L., Ramsahoye, B., Burgess, K., Cook, K., Weidt, S., Creanor, J., Harrison, D., Langridge-Smith, P., Hupp, T. & Hayward, L., 2008, In: Analytical Chemistry. 80, 11, p. 4147-4153 7 p.

  Research output: Contribution to journal › Article › peer-review

• 2007

  Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2

  Murray, E., McKenna, E. O., Burch, L. R., Dillon, J., Langridge-Smith, P., Kolch, W., Pitt, A. & Hupp, T. R., 2007, In: Biochemistry. 46, 48, p. 13742-51 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Peptide Aptamers 100%
  • Biological Marker 51%
  • Biomarkers 36%
  • Peptide 32%
  • Protein 23%

• Platination of superoxide dismutase with cisplatin: tracking the ammonia ligands using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS)

  Weidt, S. K., Mackay, C. L., Langridge-Smith, P. R. R. & Sadler, P. J., 2007, In: Chemical Communications. 17, p. 1719-21 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Superoxide Dismutase 100%
  • Cyclotron resonance 94%
  • Ligands 68%
  • Mass spectrometry 65%
  • FT/ICR Mass Spectrometry 58%
• 2006

  Destabilizing missense mutations in the tumour suppressor protein p53 enhance its ubiquitination in vitro and in vivo

  Shimizu, H., Saliba, D., Wallace, M., Finlan, L., Langridge-Smith, P. R. R. & Hupp, T. R., 2006, In: Biochemical Journal. 397, 2, p. 355-67 13 p.

  Research output: Contribution to journal › Article › peer-review

  • Tumor Suppressor Protein 100%
  • Tumor Suppressor Protein p53 84%
  • Ubiquitination 68%
  • Missense Mutation 65%
  • Mutation 53%
• 2005

  Structural investigation of naturally occurring peptides by electron capture dissociation and AMBER force field modelling

  Polfer, N. C., Haselmann, K. F., Langridge-Smith, P. R. R. & Barran, P. E., 10 Jun 2005, In: Molecular Physics. 103, 11-12, p. 1481-1489 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Electron-Capture Dissociation 100%
  • peptides 76%
  • electron capture 74%
  • field theory (physics) 70%
  • Arginine 69%

• Is it biologically relevant to measure the structures of small peptides in the gas-phase?

  Barran, P. E., Polfer, N. C., Campopiano, D. J., Clarke, D. J., Langridge-Smith, P. R. R., Langley, R. J., Govan, J. R. W., Maxwell, A., Dorin, J. R., Millar, R. P. & Bowers, M. T., 1 Feb 2005, In: International Journal of Mass Spectrometry. 240, 3, p. 273-284 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Gonadotropin-Releasing Hormone 100%
  • Peptides 82%
  • hormones 71%
  • peptides 69%
  • releasing 60%
• 2004

  Analysis of low oxidation state transition metal clusters by laser desorption/ionization time-of-flight mass spectrometry

  Dyson, P. J., Hearley, A. K., Johnson, B. F. G., Langridge-Smith, P. R. R. & McIndoe, J. S., 9 Aug 2004, In: Inorganic Chemistry. 43, 16, p. 4962-4973 12 p.

  Research output: Contribution to journal › Article › peer-review

  • metal clusters 100%
  • Laser Desorption 94%
  • Time-of-Flight Mass Spectrometry 82%
  • mass spectroscopy 80%
  • desorption 72%
• 2003

  Inert site in a protein zinc cluster: Isotope exchange by high resolution mass spectrometry

  Blindauer, C. A., Polfer, N. C., Keiper, S. E., Harrison, M. D., Robinson, N. J., Langridge-Smith, P. R. R. & Sadler, P. J., 19 Mar 2003, In: Journal of the American Chemical Society. 125, 11, p. 3226-3227 2 p.

  Research output: Contribution to journal › Article › peer-review

• A strategy for generating naked-metal clusters for gas-phase reactivity studies by FTICR-MS

  Butcher, C. P. G., Dinca, A., Dyson, P. J., Johnson, B. F. G., Langridge-Smith, P. R. R. & McIndoe, J. S., 2003, In: Angewandte Chemie International Edition. 42, 46, p. 5752-5755 4 p.

  Research output: Contribution to journal › Article › peer-review
• 2002

  Electron capture dissociation of polypeptides using a 3 Tesla Fourier transform ion cyclotron resonance mass spectrometer

  Polfer, N. C., Haselmann, K. F., Zubarev, R. A. & Langridge-Smith, P. R. R., 2002, In: Rapid communications in mass spectrometry. 16, 10, p. 936-943 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Electron-Capture Dissociation 100%
  • FT/ICR Mass Spectrometry 79%
  • Polypeptide 57%
  • Electron Particle 22%
  • Bee Venom 20%

• On the use of breakdown graphs combined with energy-dependent mass spectrometry to provide a complete picture of fragmentation processes

  Butcher, C. P. G., Dyson, P. J., Johnson, B. F. G., Langridge-Smith, P. R. R., McIndoe, J. S. & Whyte, C., 2002, In: Rapid communications in mass spectrometry. 16, 16, p. 1595-1598 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Fragmentation 100%
  • Mass Spectrometry 80%
  • Metal Carbonyl 71%
  • Collisionally Activated Dissociation (CAD) 64%
  • Collision 59%