Pat Langridge-Smith

Search results

• 2016

  Determination of Protein Thiol Reduction Potential by Isotope Labeling and Intact Mass Measurement

  Thurlow, S. E., Kilgour, D. P., Campopiano, D. J., Mackay, C. L., Langridge-Smith, P. R. R., Clarke, D. J. & Campbell, C. J., 1 Mar 2016, In: Analytical Chemistry. 88, 5, p. 2727-33 7 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Sulfhydryl Compounds
  • Isotopes
  • Labeling
  • Proteins
  • Oxidation-Reduction

• Mass spectrometry analysis of the oxidation states of the pro-oncogenic protein anterior gradient-2 reveals covalent dimerization via an intermolecular disulphide bond

  Clarke, D. J., Murray, E., Faktor, J., Mohtar, A., Vojtesek, B., MacKay, C. L., Smith, P. L. & Hupp, T. R., May 2016, In: BBA - Bioenergetics.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Dimerization
  • Mass spectrometry
  • Disulfides
  • Cysteine
  • Mass Spectrometry

• Screen-printed digital microfluidics combined with surface acoustic wave nebulization for hydrogen-deuterium exchange measurements

  Monkkonen, L., Edgar, J. S., Winters, D., Heron, S. R., Mackay, C. L., Masselon, C. D., Stokes, A. A., Langridge-Smith, P. R. R. & Goodlett, D. R., Mar 2016, In: Journal of Chromatography A. 1439, p. 161-6 6 p.

  Research output: Contribution to journal › Article › peer-review

  File

  • Deuterium Exchange Measurement
  • Digital microfluidics
  • Deuterium
  • Microfluidics
  • Surface waves
• 2015

  Nebulization of water/glycerol droplets generated by ZnO/Si surface acoustic wave devices

  Guo, Y. J., Denison, A. P., Li, Y., Luo, J., Zhu, X. T., Mackay, C. L. M., Langridge-Smith, P., Walton, A. & Fu, R., 2015, In: Microfluidics and Nanofluidics. 19, p. 273-282 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Acoustic surface wave devices
  • surface acoustic wave devices
  • Glycerol
  • glycerols
  • Water
• 2014

  A novel two-laser interface for coupling capillary electrochromatography with ion-trap time-of-flight mass spectrometry

  Simpson, D. C., Yates, A. J., Knox, J. H. & Langridge-smith, P. R. R., 15 Apr 2014, In: International Journal of Mass Spectrometry. 363, p. 8-15

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Mass spectrometry
  • mass spectroscopy
  • Ions
  • Ionization
  • Lasers

• Dissecting the Dynamic Conformations of the Metamorphic Protein Lymphotactin

  Harvey, S. R., Porrini, M., Konijnenberg, A., Clarke, D. J., Tyler, R. C., Langridge-Smith, P. R. R., MacPhee, C. E., Volkman, B. F. & Barran, P. E., 30 Oct 2014, In: Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry). 118, 43, p. 12348-59 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Conformations
  • Dimers
  • Monomers
  • Proteins
  • Mass spectrometry
• 2013

  Competition between glutathione and DNA oligonucleotides for ruthenium(II) arene anticancer complexes

  Wang, F., Xu, J., Wu, K., Weidt, S. K., Mackay, C. L., Langridge-Smith, P. R. R. & Sadler, P. J., 7 Mar 2013, In: Dalton Transactions. 42, 9, p. 3188-3195 8 p.

  Research output: Contribution to journal › Article › peer-review

• Mass Spectrometry Imaging for Dissecting Steroid Intracrinology within Target Tissues

  Cobice, D. F., Mackay, C. L., Goodwin, R. J. A., McBride, A., Langridge-Smith, P. R., Webster, S. P., Walker, B. R. & Andrew, R., 3 Dec 2013, In: Analytical Chemistry. 85, 23, p. 11576-11584 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Mass spectrometry
  • Tissue
  • Imaging techniques
  • Steroids
  • 11-dehydrocorticosterone

• Redox regulation of tumour suppressor protein p53: identification of the sites of hydrogen peroxide oxidation and glutathionylation

  Scotcher, J., Clarke, D. J., Mackay, C. L., Hupp, T., Sadler, P. J. & Langridge-Smith, P. R. R., Mar 2013, In: Chemical Science. 4, 3, p. 1257-1269 13 p.

  Research output: Contribution to journal › Article › peer-review

• The chemical basis of serine palmitoyltransferase inhibition by myriocin

  Wadsworth, J. M., Clarke, D. J., McMahon, S. A., Lowther, J. P., Beattie, A. E., Langridge-Smith, P. R. R., Broughton, H. B., Dunn, T. M., Naismith, J. H. & Campopiano, D. J., 25 Sep 2013, In: Journal of the American Chemical Society. 135, 38, p. 14276-85 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • thermozymocidin
  • Serine C-Palmitoyltransferase
  • Enzymes
  • Phosphates
  • X ray crystallography
• 2012

  An affinity purification procedure to isolate oxidized p53

  Scotcher, J., Clarke, D. J. & Langridge-Smith, P. R. R., 1 Jan 2012, In: Analytical Biochemistry. 420, 1, p. 96-98 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Purification
  • Biotin
  • Cysteine
  • Tumor Suppressor Protein p53
  • Oxidation-Reduction

• Appropriate degree of trust: deriving confidence metrics for automatic peak assignment in high-resolution mass spectrometry

  Kilgour, D. P. A., Mackay, C. L., Langridge-Smith, P. R. R. & O'Connor, P. B., 2012, In: Analytical Chemistry. 84, 17, p. 7431-5 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Mass spectrometry
  • Mass spectrometers
  • Spectrum analysis
  • Ions
  • Defects

• Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation

  Goodwin, R. J. A., Nilsson, A., Borg, D., Langridge-Smith, P. R. R., Harrison, D. J., Mackay, C. L., Iverson, S. L. & Andrén, P. E., 30 Aug 2012, In: Journal of proteomics. 75, 16, p. 4912-4920 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
  • Mountings
  • Tapes
  • Carbon
  • Animals

• Ferric ion (hydr)oxo clusters in the "Venus flytrap" cleft of FbpA: Mössbauer, calorimetric and mass spectrometric studies

  Mukherjee, A., Bilton, P. R., Mackay, L., Janoschka, A., Zhu, H., Rea, D., Langridge-Smith, P. R. R., Campopiano, D. J., Teschner, T., Trautwein, A. X., Schünemann, V. & Sadler, P. J., 2012, In: JBIC Journal of Biological Inorganic Chemistry.

  Research output: Contribution to journal › Article › peer-review

  • Droseraceae
  • Iron
  • Ions
  • Cyclotron resonance
  • Application programs

• Lactate, a product of glycolytic metabolism, inhibits histone deacetylase activity and promotes changes in gene expression

  Latham, T., Mackay, L., Sproul, D., Karim, M., Culley, J., Harrison, D. J., Hayward, L., Langridge-Smith, P., Gilbert, N. & Ramsahoye, B. H., Jun 2012, In: Nucleic Acids Research. 40, 11, p. 4794-4803 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Histone Deacetylases
  • Lactic Acid
  • Gene Expression
  • Genes
  • Histone Deacetylase Inhibitors

• L-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5 '-phosphate-thiazolidine adduct

  Lowther, J., Beattie, A. E., Langridge-Smith, P. R. R., Clarke, D. J. & Campopiano, D. J., Aug 2012, In: Medchemcomm. 3, 8, p. 1003-1008 6 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Test Structures for Characterizing the Integration of EWOD and SAW Technologies for Microfluidics

  Li, Y., Fu, R. Y., Winters, D., Flynn, B. W., Parkes, B., Brodie, D. S., Liu, Y., Terry, J., Haworth, L. I., Bunting, A. S., Stevenson, T., Smith, S., MacKay, C. L., Langridge-Smith, P. R. R., Stokes, A. A. & Walton, A. J., 3 Aug 2012, In: IEEE Transactions on Semiconductor Manufacturing. 25, 3, p. 323-330 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Surface waves
  • Microfluidics
  • Wetting
  • wetting
  • Acoustic waves

• Use of an artificial immune system derived method for the charge state assignment of small-molecule mass spectra

  Kilgour, D. P. A., Mackay, C. L., Langridge-Smith, P. R. R. & O'Connor, P. B., 2012, In: Analytical Chemistry. 84, 17, p. 7436-9 4 p.

  Research output: Contribution to journal › Article › peer-review

  • State assignment
  • Immune system
  • Molecules
  • Ions
  • Artificial intelligence
• 2011

  Identification of Two Reactive Cysteine Residues in the Tumor Suppressor Protein p53 Using Top-Down FTICR Mass Spectrometry

  Scotcher, J., Clarke, D., Weidt, S., Mackay, L., Hupp, T., Sadler, P. & Langridge-Smith, P., May 2011, In: Journal of the American Society for Mass Spectrometry. 22, 5, p. 888-897

  Research output: Contribution to journal › Article › peer-review

  • Tumor Suppressor Protein p53
  • Mass spectrometry
  • Cysteine
  • Mass Spectrometry
  • Oxidation-Reduction

• Mapping a noncovalent protein-peptide interface by top-down FTICR mass spectrometry using electron capture dissociation

  Clarke, D. J., Murray, E., Hupp, T., Mackay, C. L. & Langridge-Smith, P. R. R., 2011, In: Journal of the American Society for Mass Spectrometry. 22, 8, p. 1432-40 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Mass spectrometry
  • Mass Spectrometry
  • Electrons
  • Peptides
  • Proteins

• Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

  Goodwin, R. J. A., Pitt, A. R., Harrison, D., Weidt, S. K., Langridge-Smith, P. R. R., Barrett, M. P. & Logan Mackay, C., 2011, In: Rapid communications in mass spectrometry. 25, 7, p. 969-72 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Cyclotron resonance
  • Ionization
  • Mass spectrometry
  • Desorption
  • Fourier transforms
• 2010

  Online Quench-Flow Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Elucidating Kinetic and Chemical Enzymatic Reaction Mechanisms

  Clarke, D. J., Stokes, A., Langridge-Smith, P. & Mackay, C. L., 1 Mar 2010, In: Analytical Chemistry. 82, 5, p. 1897-1904 8 p.

  Research output: Contribution to journal › Article › peer-review

• Subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins based on catalytic activity

  Clarke, D. J., Ortega, X. P., Mackay, C. L., Valvano, M. A., Govan, J. R. W., Campopiano, D. J., Langridge-Smith, P. & Brown, A. R., 2010, In: Biochemistry. 49, 6, p. 1319-30 12 p.

  Research output: Contribution to journal › Article › peer-review

  • bacterioferritin
  • Burkholderia cenocepacia
  • Peroxiredoxins
  • Catalyst activity
  • Bacterial Proteins

• Top-down protein sequencing by CID and ECD using desorption electrospray ionisation (DESI) and high-field FTICR mass spectrometry

  Stokes, A. A., Clarke, D. J., Weidt, S., Langridge-Smith, P. & Mackay, C. L., 1 Jan 2010, In: International Journal of Mass Spectrometry. 289, 1, p. 54-57 4 p.

  Research output: Contribution to journal › Article › peer-review
• 2009

  Interrogating the molecular details of the peroxiredoxin activity of the Escherichia coli bacterioferritin comigratory protein using high-resolution mass spectrometry

  Clarke, D. J., Mackay, C. L., Campopiano, D. J., Langridge-Smith, P. & Brown, A. R., May 2009, In: Biochemistry. 48, 18, p. 3904-14 11 p.

  Research output: Contribution to journal › Article › peer-review

  • bacterioferritin
  • Peroxiredoxins
  • Mass spectrometry
  • Escherichia coli
  • Mass Spectrometry

• Precursor ion independent algorithm for top-down shotgun proteomics

  Tsai, Y., Scherl, A., Shaw, J., Mackay, L., Schaffer, S., Langridge-Smith, P. & Goodlett, D., 2009, In: Journal of the American Society for Mass Spectrometry. 20, 11, p. 2154-2166

  Research output: Contribution to journal › Article › peer-review

  • Proteomics
  • Firearms
  • Ions
  • Protein Precursors
  • Proteins
• 2008

  Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography Fourier transform ion cyclotron mass spectrometry combined with (18)O-labeling

  Wang, F., Weidt, S., Xu, J., Mackay, C. L., Langridge-Smith, P. R. R. & Sadler, P. J., 2008, In: Journal of the American Society for Mass Spectrometry. 19, 4, p. 544-549 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Ruthenium
  • Liquid chromatography
  • Cyclotrons
  • Mass spectrometry
  • Fourier Analysis

• Sensitive, specific, and quantitative FTICR mass spectrometry of combinatorial post-translational modifications in intact histone H4

  Mackay, C. L., Ramsahoye, B., Burgess, K., Cook, K., Weidt, S., Creanor, J., Harrison, D., Langridge-Smith, P., Hupp, T. & Hayward, L., 2008, In: Analytical Chemistry. 80, 11, p. 4147-4153 7 p.

  Research output: Contribution to journal › Article › peer-review

• Sensitive, Specific and Quantitative FT-ICR Mass Spectrometry of Combinatorial Post-Translational Modifications in Intact Histone H4

  Mackay, L., Ramsahoye, B., Burgess, K., Cook, K., Weidt, S., Creanor, J., Harrison, D., Langridge-Smith, P., Hupp, T. & Hayward, L., Jun 2008, In: Analytical Chemistry. 80, 11, p. 4147-4153

  Research output: Contribution to journal › Article › peer-review

  • Mass spectrometry
  • Cyclotron resonance
  • Histones
  • Fourier transforms
  • Chromatin
• 2007

  Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2

  Murray, E., McKenna, E. O., Burch, L. R., Dillon, J., Langridge-Smith, P., Kolch, W., Pitt, A. & Hupp, T. R., 2007, In: Biochemistry. 46, 48, p. 13742-51 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Peptide Aptamers
  • Biomarkers
  • Assays
  • Microarrays
  • Biopsy

• Platination of superoxide dismutase with cisplatin: tracking the ammonia ligands using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS)

  Weidt, S. K., Mackay, C. L., Langridge-Smith, P. R. R. & Sadler, P. J., 2007, In: Chemical Communications. 17, p. 1719-21 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Superoxide Dismutase
  • Cyclotron resonance
  • Ligands
  • Mass spectrometry
  • Ammonia
• 2006

  Destabilizing missense mutations in the tumour suppressor protein p53 enhance its ubiquitination in vitro and in vivo

  Shimizu, H., Saliba, D., Wallace, M., Finlan, L., Langridge-Smith, P. R. R. & Hupp, T. R., 2006, In: Biochemical Journal. 397, 2, p. 355-67 13 p.

  Research output: Contribution to journal › Article › peer-review

  • Tumor Suppressor Protein p53
  • Oncogene Proteins
  • Ubiquitination
  • Molecular mass
  • Missense Mutation
• 2005

  Is it biologically relevant to measure the structures of small peptides in the gas-phase?

  Barran, P. E., Polfer, N. C., Campopiano, D. J., Clarke, D. J., Langridge-Smith, P. R. R., Langley, R. J., Govan, J. R. W., Maxwell, A., Dorin, J. R., Millar, R. P. & Bowers, M. T., 1 Feb 2005, In: International Journal of Mass Spectrometry. 240, 3, p. 273-284 12 p.

  Research output: Contribution to journal › Article › peer-review

• Structural investigation of naturally occurring peptides by electron capture dissociation and AMBER force field modelling

  Polfer, N. C., Haselmann, K. F., Langridge-Smith, P. R. R. & Barran, P. E., 10 Jun 2005, In: Molecular Physics. 103, 11-12, p. 1481-1489 9 p.

  Research output: Contribution to journal › Article › peer-review
• 2004

  Analysis of low oxidation state transition metal clusters by laser desorption/ionization time-of-flight mass spectrometry

  Dyson, P. J., Hearley, A. K., Johnson, B. F. G., Langridge-Smith, P. R. R. & McIndoe, J. S., 9 Aug 2004, In: Inorganic Chemistry. 43, 16, p. 4962-4973 12 p.

  Research output: Contribution to journal › Article › peer-review
• 2003

  A strategy for generating naked-metal clusters for gas-phase reactivity studies by FTICR-MS

  Butcher, C. P. G., Dinca, A., Dyson, P. J., Johnson, B. F. G., Langridge-Smith, P. R. R. & McIndoe, J. S., 2003, In: Angewandte Chemie International Edition. 42, 46, p. 5752-5755 4 p.

  Research output: Contribution to journal › Article › peer-review

• Inert site in a protein zinc cluster: Isotope exchange by high resolution mass spectrometry

  Blindauer, C. A., Polfer, N. C., Keiper, S. E., Harrison, M. D., Robinson, N. J., Langridge-Smith, P. R. R. & Sadler, P. J., 19 Mar 2003, In: Journal of the American Chemical Society. 125, 11, p. 3226-3227 2 p.

  Research output: Contribution to journal › Article › peer-review
• 2002

  Electron capture dissociation of polypeptides using a 3 Tesla Fourier transform ion cyclotron resonance mass spectrometer

  Polfer, N. C., Haselmann, K. F., Zubarev, R. A. & Langridge-Smith, P. R. R., 2002, In: Rapid communications in mass spectrometry. 16, 10, p. 936-943 8 p.

  Research output: Contribution to journal › Article › peer-review

• On the use of breakdown graphs combined with energy-dependent mass spectrometry to provide a complete picture of fragmentation processes

  Butcher, C. P. G., Dyson, P. J., Johnson, B. F. G., Langridge-Smith, P. R. R., McIndoe, J. S. & Whyte, C., 2002, In: Rapid communications in mass spectrometry. 16, 16, p. 1595-1598 4 p.

  Research output: Contribution to journal › Article › peer-review