An investigation into the kinetic properties of NMDA receptors using chimaeric channel constructs.

Project Details

Layman's description

NMDA receptors are a class of specialized protiens activated by the neurotransmitter, glutamate. They play key roles in both physiological and pathophysiological brain function. There are four main types of NMDA receptor each with unique properties and this project assess how different regions of the control controlled particular aspects of these properties.

Key findings

Through a combination of site-directed mutagenesis and use of selective agonists, partial agonists and antagonists the roles played by indvidual amino acids in the ligand binding site if various NMDAR subunits were identfied. Quantitative descripitons of agonist, partial agonist and antagonist actions were eluciidated by use of kinetic modelling. Chimeric subunit constructs were generated to demonstrate that NMDAR subunits are modular in their design and that their unique properties could be transferred between subunits by swapping such domains.
StatusFinished
Effective start/end date1/10/0531/01/09

Funding

  • BBSRC: £128,098.00