Genomic analysis of complex speciation in heliconius

  • Blaxter, Mark (Principal Investigator)
  • Jiggins, Christopher (Researcher)

Project Details

Description

This project aimed to describe and define the genetic and genomic underpinnings of the radiation of the Heliconius group of butterflies in Central and South America.

Layman's description

An international collaborative study to map the genome of a South American butterfly has identified the secret behind its mimetic nature.
The genome sequence of the Postman butterfly, Heliconius melpomene, was used as a reference to study species that live together in the Peruvian Amazon. Several of these share bright wing patterns in order to reinforce a warning signal that they are bad to eat - dissuading predators from attack.
Sequence data showed that those species with similar wing patterns shared a similar genetic signal, precisely in those narrow regions of the genome responsible for controlling patterns. This is a result of hybrid exchange of genes between three co-mimic species, which has allowed them to copy each other’s wing patterns.
Dr Chris Jiggins of the University of Cambridge said, “These butterflies, in order to better survive in the wild, have taken successful survival techniques from other butterflies and have incorporated them into their own genetic code. This is significant as it has occurred in a natural adaptive radiation, suggesting an important role for hybrids in the origins of biodiversity.”
The phenomenon, known as adaptive introgression, involves different species sharing genetic material and has been considered very rare, especially in animals. Although many species can interbreed in the wild, the resulting hybrids are often infertile and considered an evolutionary dead-end. However, occasionally hybrids might introduce useful genetic material that can help populations adapt to changing conditions. This source of novelty might be more effective than having to wait for a mutation to occur in order to yield a similar result. In the case of butterflies, the effects can be clearly seen on their wings.
Dr Kanchon Dasmahapatra, who worked with Professor James Mallet at University College London added, “What we show is that one butterfly species can gain its protective colour pattern genes ready-made from a different species by hybridizing (or interbreeding) with it. A much faster process than having to evolve one's colour patterns from scratch.”
The butterfly genus Heliconius is found in the tropical and sub-tropical regions of the Americas, from the Amazon basin to Texas and has been studied by scientists since the Victorian era.
Dr Jiggins added: “The genus Heliconius has been the subject of evolutionary studies since Darwin's time, and the original formulation of mimicry theory. We are especially interested in them because their convergent wing patterns offer an opportunity to study the repeatability of evolutionary change.”
The sequencing of a butterfly genome also offers some remarkable insights into the biology of these charismatic insects. Butterflies are thought to be more visual in their communication as compared to night-flying moths. Surprisingly, in work done by Adriana Briscoe at the University of California, analysis carried out at the University of California by Adriana Briscoe showed that they have an even greater array of genes involved in chemical communication as compared to moths.
Owen McMillan of the Smithsonian Tropical Research Institute in Panama said: “We are accustomed to thinking of butterflies as brightly coloured, primarily visual insects, but the presence of such a rich array of olfactory receptors and chemosensory genes, suggests that smell and taste are also vital.”
The study heralds a new era in genome biology, in which genome sequencing has become available to small groups of researchers for their own organism of choice. In this case academics from 9 labs across the globe each contributed to a pool of funding that permitted the sequencing effort, without any major grant funding.
Dr Jiggins added, “I think it’s been a great privilege to work with all these people across the world, and especially our collaborators in Latin America, who are all equally excited about these butterflies.”

Key findings

The evolutionary importance of hybridization and introgression has
long been debated1. Hybrids are usually rare and unfit, but even
infrequent hybridization can aid adaptation by transferring beneficial
traits between species. Here we use genomic tools to investigate
introgression inHeliconius, a rapidly radiating genus of neotropical
butterflies widely used in studies of ecology, behaviour, mimicry and
speciation2–5. We sequenced the genome of Heliconius melpomene
and compared it with other taxa to investigate chromosomal evolution
in Lepidoptera and gene flow among multiple Heliconius
species and races. Among 12,669 predicted genes, biologically
important expansions of families of chemosensory and Hox
genes are particularly noteworthy. Chromosomal organization
has remained broadly conserved since the Cretaceous period, when
butterflies split from the Bombyx (silkmoth) lineage. Using
genomic resequencing, we show hybrid exchange of genes between
three co-mimics, Heliconius melpomene, Heliconius timareta and
Heliconius elevatus, especially at two genomic regions that control
mimicry pattern. We infer that closely related Heliconius species
exchange protective colour-pattern genes promiscuously, implying
that hybridization has an important role in adaptive radiation.
StatusFinished
Effective start/end date1/07/0930/06/12

Funding

  • BBSRC: £130,606.00
  • Formin is Associated with Left-Right Asymmetry in the Pond Snail and the Frog

    Davison, A., McDowell, G. S., Holden, J. M., Johnson, H. F., Koutsovoulos, G. D., Liu, M. M., Hulpiau, P., Van Roy, F., Wade, C. M., Banerjee, R., Yang, F., Chiba, S., Davey, J. W., Jackson, D. J., Levin, M. & Blaxter, M. L., 7 Mar 2016, In: Current biology : CB. 26, 5, p. 654-660 7 p.

    Research output: Contribution to journalArticlepeer-review

    Open Access
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  • Genome-wide patterns of divergence and gene flow across a butterfly radiation

    Nadeau, N. J., Martin, S. H., Kozak, K. M., Salazar, C., Dasmahapatra, K. K., Davey, J. W., Baxter, S. W., Blaxter, M. L., Mallet, J. & Jiggins, C. D., Feb 2013, In: Molecular Ecology. 22, 3, p. 814-826 13 p.

    Research output: Contribution to journalArticlepeer-review

  • Butterfly genome reveals promiscuous exchange of mimicry adaptations among species

    The Heliconius Genome Consortium, Dasmahapatra, K. K., Walters, J. R., Briscoe, A. D., Davey, J. W., Whibley, A., Nadeau, N. J., Zimin, A. V., Hughes, D. S. T., Ferguson, L. C., Martin, S. H., Salazar, C., Lewis, J. J., Adler, S., Ahn, S-J., Baker, D. A., Baxter, S. W., Chamberlain, N. L., Chauhan, R., Counterman, B. A. & 31 others, Dalmay, T., Gilbert, L. E., Gordon, K., Heckel, D. G., Hines, H. M., Hoff, K. J., Holland, P. W. H., Jacquin-Joly, E., Jiggins, F. M., Jones, R. T., Kapan, D. D., Kersey, P., Lamas, G., Lawson, D., Mapleson, D., Maroja, L. S., Martin, A., Moxon, S., Palmer, W. J., Papa, R., Papanicolaou, A., Pauchet, Y., Ray, D. A., Rosser, N., Salzberg, S. L., Supple, M. A., Eland, C., Gharbi, K., Thomson, M., Blaxter, M. L. & Joron, M., Jul 2012, In: Nature. 487, 7405, p. 94-98 5 p.

    Research output: Contribution to journalArticlepeer-review

    Open Access
    File