Investigation of the role of the primary cilia and centrosome during signalling events in development using the chicken mutant TALPID3: Fellowship Dr M Davey

Project Details

Layman's description

Chicken and mouseEmbryos which lack functional TALPID3 protein die during development.

Key findings

TALPID3 protein is a centrosomal protein
It is postulated through analysis of TALPID3 homologues that TALPID3 has a Eumetazoan origin
TALPID3 has 2 essential coiled coiled domains
TALPID3 has 1 essential region of intrisic disorder
TALPID3 is essential for motile and non-motile cilia development
TALPID3 is essential for migration and orientation of centrosomes during ciliogenesis
TALPID3 is not essential for centriol duplication
TALPID3 is not essential for axoneme formation
Telencephalic choroid plexus forms in in talpid3-/- chicken embryos
Lung development is abnormal in talpid3-/- and phenocopies human ciliopathy syndromes
Liver development is abnormal in talpid3-/- and phenocopies human ciliopathy syndromes
Inner ear development is abnormal in talpid3-/- and phenocopies human ciliopathy syndromes
talpid3-/- embryos have planar cell polarity type anatomical defects
talpid3-/- embryos/cells have intracellular mislocalisation of core planar cell polarity proteins
TALPID3 is epistatic to KIF7
StatusFinished
Effective start/end date1/07/0828/02/14

Funding

  • BBSRC: £1,378,066.00