SIRNA: A new technology for investigating gene function in developing organs.

Application of siRNA molecules is a viable way of controlling gene function in organs developing in vitro, and we have demonstrated its use to untangle a long-standing mystery about the tumour suppressor WT1 (see Davies et al. 2004). Our work in this project identified some serious limitations and set the foundations for later projects that found ways of removing these limitations: see in particular Unbekandt and Davies, and the more recent reviews, listed below.
We have used the technology developed in this project for the last 6-years (ie since the project ended) to address a number of biological problems, working with a variety of collaborators in the UK and internationally. Some of the publications arising from these collaborations are listed below.