Using targets of AS1 to dissect developmental control in the shoot apex

Project Details

Description

The ASYMMETRIC LEAVES1 (AS1) transcription factor is active only in developing leaves and needed for normal leaf development. The aim of this project was to understand how AS1 controls leaf formation by identifying the genes that it regulates - using both genetic and biochemical approaches.

Layman's description

The ASYMMETRIC LEAVES1 (AS1) protein is active only in developing leaves and needed for normal leaf development. Biochemically, it functions as a transcription factor, regulating gene expression. This project aimed to gain insights into the control of leaf formation by identifying the genes that AS1 regulates using both genetic and biochemical techniques.

Key findings

Genetic screens showed that a family of proteins, known to be involved in leaf growth and polarity, are repressed transcriptionally by AS1 in the upper (adaxial) part of the leaf and that repression also occurs at the level of protein degradation.
AS1 was also found to repress genes that defend plants against necrotrophic pathogens, such as the grey mold Botrytis and are induced by methyl jasmonate signals on attack by the pathogen. This was shown to involve binding of the AS1 protein to defence gene promoters. Different plant species lacking AS1 activity were found to be resistant to a range of necrotrophic pathogens.
StatusFinished
Effective start/end date17/11/0316/11/06

Funding

  • BBSRC: £177,761.00

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