α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in

Oliver Gordon; Conor M Henry; Naren Srinivasan; Susan Ahrens; Anna Franz; Safia Deddouche; Probir Chakravarty; David Phillips; Roger George; Svend Kjaer; David Frith; Ambrosius P Snijders; Rita S Valente; Carolina J Simoes da Silva; Luis Teixeira; Barry Thompson; Marc S Dionne; Will Wood; Caetano Reis E Sousa

doi:10.7554/eLife.38636

α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in

Oliver Gordon, Conor M Henry, Naren Srinivasan, Susan Ahrens, Anna Franz, Safia Deddouche, Probir Chakravarty, David Phillips, Roger George, Svend Kjaer, David Frith, Ambrosius P Snijders, Rita S Valente, Carolina J Simoes da Silva, Luis Teixeira, Barry Thompson, Marc S Dionne, Will Wood, Caetano Reis E Sousa

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Abstract / Description of output

Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent genes (<xref ref-type="bibr" rid="bib10">Srinivasan et al., 2016</xref>). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila.

Original language	English
Journal	eLIFE
Volume	7
Early online date	27 Sept 2018
DOIs	https://doi.org/10.7554/eLife.38636
Publication status	Published - 3 Oct 2018

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10.7554/eLife.38636

elife-38636-v2Final published version, 1.13 MBLicence: Creative Commons: Attribution (CC-BY)

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Gordon, O., Henry, C. M., Srinivasan, N., Ahrens, S., Franz, A., Deddouche, S., Chakravarty, P., Phillips, D., George, R., Kjaer, S., Frith, D., Snijders, A. P., Valente, R. S., Simoes da Silva, C. J., Teixeira, L., Thompson, B., Dionne, M. S., Wood, W., & Reis E Sousa, C. (2018). α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in. eLIFE, 7. Advance online publication. https://doi.org/10.7554/eLife.38636

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title = "α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in",

abstract = "Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila.",

author = "Oliver Gordon and Henry, {Conor M} and Naren Srinivasan and Susan Ahrens and Anna Franz and Safia Deddouche and Probir Chakravarty and David Phillips and Roger George and Svend Kjaer and David Frith and Snijders, {Ambrosius P} and Valente, {Rita S} and {Simoes da Silva}, {Carolina J} and Luis Teixeira and Barry Thompson and Dionne, {Marc S} and Will Wood and {Reis E Sousa}, Caetano",

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year = "2018",

month = oct,

day = "3",

doi = "10.7554/eLife.38636",

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Gordon, O, Henry, CM, Srinivasan, N, Ahrens, S, Franz, A, Deddouche, S, Chakravarty, P, Phillips, D, George, R, Kjaer, S, Frith, D, Snijders, AP, Valente, RS, Simoes da Silva, CJ, Teixeira, L, Thompson, B, Dionne, MS, Wood, W & Reis E Sousa, C 2018, 'α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in', eLIFE, vol. 7. https://doi.org/10.7554/eLife.38636

TY - JOUR

T1 - α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in

AU - Gordon, Oliver

AU - Henry, Conor M

AU - Srinivasan, Naren

AU - Ahrens, Susan

AU - Franz, Anna

AU - Deddouche, Safia

AU - Chakravarty, Probir

AU - Phillips, David

AU - George, Roger

AU - Kjaer, Svend

AU - Frith, David

AU - Snijders, Ambrosius P

AU - Valente, Rita S

AU - Simoes da Silva, Carolina J

AU - Teixeira, Luis

AU - Thompson, Barry

AU - Dionne, Marc S

AU - Wood, Will

AU - Reis E Sousa, Caetano

PY - 2018/10/3

Y1 - 2018/10/3

N2 - Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila.

AB - Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila.

U2 - 10.7554/eLife.38636

DO - 10.7554/eLife.38636

M3 - Article

C2 - 30260317

SN - 2050-084X

VL - 7

JO - eLIFE

JF - eLIFE

ER -

α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in

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