γδ T cells control murine skin inflammation and subcutaneous adipose wasting during chronic Trypanosoma brucei infection

Juan F. Quintana*, Matthew C. Sinton, Praveena Chandrasegaran, Agatha Nabilla Lestari, Rhiannon Heslop, Bachar Cheaib, John Ogunsola, Dieudonne Mumba Ngoyi, Nono-Raymond Kuispond Swar, Anneli Cooper, Neil Mabbott, Seth B. Coffelt, Annette MacLeod*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

African trypanosomes colonise the skin to ensure parasite transmission. However, how the skin responds to trypanosome infection remains unresolved. Here, we investigate the local immune response of the skin in a murine model of infection using spatial and single cell transcriptomics. We detect expansion of dermal IL-17A-producing Vγ6 + cells during infection, which occurs in the subcutaneous adipose tissue. In silico cell-cell communication analysis suggests that subcutaneous interstitial preadipocytes trigger T cell activation via Cd40 and Tnfsf18 signalling, amongst others. In vivo, we observe that female mice deficient for IL-17A-producing Vγ6 + cells show extensive inflammation and limit subcutaneous adipose tissue wasting, independently of parasite burden. Based on these observations, we propose that subcutaneous adipocytes and Vγ6 + cells act in concert to limit skin inflammation and adipose tissue wasting. These studies provide new insights into the role of γδ T cell and subcutaneous adipocytes as homeostatic regulators of skin immunity during chronic infection.

Original languageEnglish
Article number5279
Pages (from-to)1-17
Number of pages17
JournalNature Communications
Volume14
Issue number29
Early online date29 Aug 2023
DOIs
Publication statusPublished - 29 Aug 2023

Keywords / Materials (for Non-textual outputs)

  • African trypanosomes
  • γδ T cells
  • Vγ6+ 23 cells
  • adipocytes skin inflammation.skin inflammation.
  • skin inflammation

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