Projects per year
The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammatory responses and are protected against the adverse metabolic effects of a high fat diet. However, the influence of 11β-HSD1 on the composition of the gut microbiome has not previously been investigated. We used high-throughput 16S rDNA amplicon sequencing to characterize the gut microbiome of 11β-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a cholesterol and fat-enriched 'western' diet. 11β-HSD1 deficiency significantly altered the composition of the gut microbiome, and did so in a diet-specific manner. On a western diet, 11β-HSD1 deficiency increased the relative abundance of the family Bacteroidaceae, and on a chow diet, it altered relative abundance of the family Prevotellaceae. Our results demonstrate that i) genetic effects on host-microbiome interactions can depend upon diet, and ii) that alterations in the composition of the gut microbiome may contribute to aspects of the metabolic and/or inflammatory phenotype observed with 11β-HSD1-deficiency.
|Journal||Journal of Endocrinology|
|Early online date||24 Nov 2016|
|Publication status||Published - Feb 2017|
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- 3 Finished
Future-Proofing the sustainability of the MRC high throughput sequencing hub in Scotland
1/10/12 → 30/09/14
Watson, M., Archibald, A. & Burt, D.
1/04/12 → 31/03/17
NERC Molecular Genetics Facility core grant
1/04/08 → 31/03/11
- Centre for Cardiovascular Science
- Deanery of Clinical Sciences - UoE Retired Staff
Person: Academic: Research Active , Affiliated Independent Researcher