11-BETA-HYDROXYSTEROID DEHYDROGENASE IN THE RAT OVARY - HIGH EXPRESSION IN THE OOCYTE

R BENEDIKTSSON, J L W YAU, S LOW, L P BRETT, B E COOKE, C R W EDWARDS, J R SECKL

Research output: Contribution to journalArticlepeer-review

Abstract

The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyses the conversion of physiological glucocorticoids to inactive products, thus modifying the access of glucocorticoids to glucocorticoid and miner-alocorticoid receptors. Glucocorticoids may affect ovarian function both indirectly and via binding to ovarian receptors. We have demonstrated 11 beta-HSD bioactivity and mRNA expression in rat ovary in vitro. The enzyme was localized to oocytes and luteal bodies immunohistochemically using two antibodies raised against purified rat liver 11 beta-HSD. These data are supported by in-situ hybridization studies, which also localized 11 beta-HSD mRNA expression to oocytes and luteal bodies. The results suggest that 11 beta-HSD may modulate the effects of glucocorticoid on ovarian function.

Original languageEnglish
Pages (from-to)53
Number of pages0
JournalJournal of Endocrinology
Volume135
Issue number1
Publication statusPublished - Oct 1992

Cite this