11-BETA-HYDROXYSTEROID DEHYDROGENASE MESSENGER-RNA EXPRESSION IN RAT-KIDNEY

J L W YAU, A D VANHAARST, M P MOISAN, S FLEMING, C R W EDWARDS, J R SECKL

Research output: Contribution to journalComment/debatepeer-review

Abstract

11-beta-Hydroxysteroid dehydrogenase (11-beta-OHSD) protects non-specific renal mineralocorticoid receptors from exposure to circulating glucocorticoid in vivo by catalyzing the conversion of corticosterone to inactive 11-dehydrocorticosterone. Although 11-beta-OHSD bioactivity and aldosterone binding sites are found in distal tubular cells, mineralocorticoid receptor and 11-beta-OHSD immunoreactivities are not colocalized. However, there are several kidney isoforms of 11-beta-OHSD, not all of which may be immunoreactive, whereas only a single mRNA species has been described. Using in situ hybridization we found 11-beta-OHSD mRNA is highly expressed in all renal tubular epithelia in the rat. It is therefore likely that 11-beta-OHSD is colocalized with mineralocorticoid receptors in distal tubular cells.

Original languageEnglish
Pages (from-to)F764-F767
Number of pages4
JournalAJP - Endocrinology and Metabolism
Volume260
Issue number5
Publication statusPublished - May 1991

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