11BETA-HYDROXYSTEROID DEHYDROGENASE MESSENGER-RIBONUCLEIC-ACID EXPRESSION, BIOACTIVITY AND IMMUNOREACTIVITY IN RAT CEREBELLUM

M P MOISAN, J R SECKL, L P BRETT, C MONDER, A K AGARWAL, P C WHITE, C R W EDWARDS

Research output: Contribution to journalArticlepeer-review

Abstract

11-beta-Hydroxysteroid dehydrogenase (11-beta-OHSD) metabolizes corticosterone to inactive 11-dehydrocorticosterone and thus protects non-specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11-beta-OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11-beta-OHSD bioactivity with similar co-substrate requirements and inhibition kinetics to the renal enzyme. 11-beta-OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11-beta-OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalJournal of Neuroendocrinology
Volume2
Issue number6
Publication statusPublished - 1990

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