1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle

S. G. Rhodes, L.A. Terry, Jayne Hope, R. G. Hewinson, H.M. Vordermeier

Research output: Contribution to journalArticlepeer-review

Abstract

This report describes the presence and activity of 1,25-dihydroxyvitamin D3 (1,25-D3) in experimental bovine tuberculosis. Animals that went on to develop tuberculous lesions exhibited a rapid transient increase in serum 1,25-D3 within the first 2 weeks following infection with Mycobacterium bovis. 1,25-D3-positive mononuclear cells were later identified in all tuberculous granulomas by immunohistochemical staining of postmortem lymph node tissue. These results suggest a role for 1,25-D3 both at the onset of infection and in the development of the granuloma in these infected animals. Using a monoclonal antibody to the vitamin D receptor (VDR) as a VDR agonist, we confirmed that activation of the vitamin D pathway profoundly depresses antigen-specific, but not mitogenic, bovine peripheral blood T-cell responses (proliferation and gamma interferon production). Investigation of the mechanism of this suppression showed that the VDR antibody modified the expression of CD80 by accessory cells, such that a significant positive correlation between T-cell proliferation and accessory cell CD80 emerged.
Original languageEnglish
Pages (from-to)1129-35
Number of pages7
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number6
Publication statusPublished - Nov 2003

Keywords

  • Animals
  • Antigens, CD80/analysis
  • Calcitriol/analysis
  • Calcitriol/physiology
  • Cattle
  • Granuloma/etiology
  • Leukocytes, Mononuclear/chemistry
  • Lymph Nodes/pathology
  • Lymphocyte Activation/immunology
  • Mycobacterium bovis
  • T-Lymphocytes/immunology
  • Tuberculosis, Bovine/metabolism

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