14-3-3 regulation of Ncd reveals a new mechanism for targeting 4 proteins to the spindle in oocytes

Robin Beaven, Ricardo Correia Nunes Bastos, Christos Spanos, Pierre Rome, Cora Fiona Cullen, Juri Rappsilber, Regis Giet, Gohta Goshima, Hiroyuki Ohkura

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The meiotic spindle is formed without centrosomes in a large volume of oocytes. Local activation of crucial spindle proteins around chromosomes is important for formation and maintenance of a bipolar spindle in oocytes. We found that the phospho-docking 14-3-3 proteins stabilise spindle bipolarity in Drosophila oocytes. A critical 14-3-3 target is the minus-end directed motor Ncd (human HSET; kinesin-14) which has well documented roles in stabilising a bipolar spindle in oocytes. Phospho-docking by 14-3-3 inhibits the microtubule binding activity of the non-motor Ncd tail. Further phosphorylation by Aurora B kinase can release Ncd from this inhibitory effect of 14-3-3. As Aurora B localises to chromosomes and spindles, 14-3-3 facilitates specific association of Ncd with spindle microtubules by preventing Ncd from binding to non-spindle microtubules in oocytes. Therefore, 14-3-3 translates a spatial cue provided by Aurora B to target Ncd selectively to the spindle within the large volume of oocytes.
Original languageEnglish
Pages (from-to)3029-3039
Number of pages11
JournalJournal of Cell Biology
Issue number10
Early online date31 Aug 2017
Publication statusPublished - 2 Oct 2017


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