1D-IEF ANALYSIS OF BOLA CLASS-I EXPRESSION USING ALLO-ANTISERA REVEALS ADDITIONAL COMPLEXITY

SWK ALMURRANI*, EJ GLASS, JL WILLIAMS, RA OLIVER

*Corresponding author for this work

Research output: Contribution to journalComment/debatepeer-review

Abstract

The use of the bovine allo-antisera in lymphocyte microcytotoxicity assays suggests that there is a single highly polymorphic class I product expressed by the BoLA system encoded by one locus. In contrast, biochemical techniques, such as 1D-IEF, reveal a complex pattern of bands for BoLA class I molecules from each animal. In order to understand the origins of this heterogeneity bovine allo-antisera were used in the immunoprecipitation step of 1D-IEF and the results compared with those from immunoprecipitation using the monoclonal antibody W6/32.

By modifying existing protocols to include Gammabind G a range of bovine allo-antisera were used successfully to immunoprecpitate bovine MHC class I molecules. The results indicate that the bovine allo-antisera do not recognize all molecules previously assigned to BoLA class I serotypes by 1D-IEF. Furthermore, some of the allo-antisera immunoprecipitated molecules are not recognized by W6/32 and vice versa. This suggests that more than one polymorphic locus is expressed from the bovine MHC and that each allo-antiserum recognizes molecules encoded by different loci. Examination of the results also suggests the existence of linkage disequilibrium in the BoLA class I region.

Original languageEnglish
Pages (from-to)427-431
Number of pages5
JournalAnimal Genetics
Volume24
Issue number6
Publication statusPublished - Dec 1993

Keywords

  • BOLA
  • 1D-IEF
  • ALLOANTISERA
  • MAJOR HISTOCOMPATIBILITY COMPLEX
  • LYMPHOCYTE ANTIGENS BOLA
  • JOINT REPORT
  • BOVINE
  • CELLS
  • CATTLE
  • GENES
  • MOLECULES
  • WORKSHOP
  • BREEDS

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