Abstract
D-[2-3H]Mannose was tested as a precursor for the labelling of L-galactose residues in cell wall polysaccharides where D-galactose residues predominate. Cultured cells of spinach, rose and maize rapidly took up [2-3H]mannose (e.g. 50 % in 4–8 min). About 90 % of the 3H supplied was converted to 3H2O, the remaining 10 % being incorporated into polymers. Of the 3H incorporated into spinach polymers, 43 % was recovered in D-mannose, 37 % in L-fucose and 5 % in L-galactose residues. D-Galactose residues were not labelled. The L-[3H]galactose residues appeared to be in the pyranose form, and were released from the polymer in the form of short oligosaccharides upon treatment with «Driselase». L-[3H]Galactose residues were found in the phenol/acetic acid/H20 soluble, cold water soluble, pectic and hemicellulosic fractions of the total cell polymers, and were thus not confined to any one particular polymer. The D-galactose:L-galactose ratio in spinach polymers was ca. 70:1.
Original language | English |
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Pages (from-to) | 484-490 |
Number of pages | 7 |
Journal | Journal of Plant Physiology |
Volume | 132 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Jan 1988 |
Keywords / Materials (for Non-textual outputs)
- BAW
- BEW
- butan-1-ol/acetic acid/water (12:3:5, v/v/v)
- butan-1-ol/ethanol/ water (20: 5: 11, v/v/v)
- cell walls
- EAW
- EPW
- ethyl acetate/acetic acid/water (10: 5 : b, v/v/v)
- ethyl acetate/pyridine/water (8:2: 1, v/v/v)
- gel-permeation chromatography
- glycoprotein
- GPC
- L-fucose
- L-galactose
- oligosaccharide
- paper chromatography
- PC
- phenol/acetic acid/water (2:1:1, w/v/v)
- polysaccharide
- Rosa sp. («Paul's Scarlet»)
- Spinacia oleracea
- TFA
- trifluoroacetic acid
- water-saturated phenol
- Zea mays
- [2-H]mannose
- φAW
- φW