TY - JOUR
T1 - 2,6-diaminopurine promotes repair of DNA lesions under prebiotic conditions
AU - Szabla, Rafał
AU - Zdrowowicz, Magdalena
AU - Spisz, Paulina
AU - Green, Nicholas J.
AU - Stadlbauer, Petr
AU - Kruse, Holger
AU - Šponer, Jiří
AU - Rak, Janusz
N1 - Funding Information:
The authors thank Prof. John D. Sutherland, Prof. Dimitar D. Sasselov, and Dr. Corinna Kufner for helpful discussions. This research was supported by the Simons Foundation (494188 to R.S.), the Foundation for Polish Science (START Fellowship to R.S.), the Czech Science Foundation (project 21-23718S to J.S. and P.St.), the project SYMBIT (reg. number: CZ.02.1.01/0.0/0.0/15_003/0000477 to J.S. and H.K.) financed by the ERDF and a grant from the Ministry of Science and Higher Education in Poland (DS/531-T080-D494-20 to J.R.).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/5/21
Y1 - 2021/5/21
N2 - High-yielding and selective prebiotic syntheses of RNA and DNA nucleotides involve UV irradiation to promote the key reaction steps and eradicate biologically irrelevant isomers. While these syntheses were likely enabled by UV-rich prebiotic environment, UV-induced formation of photodamages in polymeric nucleic acids, such as cyclobutane pyrimidine dimers (CPDs), remains the key unresolved issue for the origins of RNA and DNA on Earth. Here, we demonstrate that substitution of adenine with 2,6-diaminopurine enables repair of CPDs with yields reaching 92%. This substantial self-repairing activity originates from excellent electron donating properties of 2,6-diaminopurine in nucleic acid strands. We also show that the deoxyribonucleosides of 2,6-diaminopurine and adenine can be formed under the same prebiotic conditions. Considering that 2,6-diaminopurine was previously shown to increase the rate of nonenzymatic RNA replication, this nucleobase could have played critical roles in the formation of functional and photostable RNA/DNA oligomers in UV-rich prebiotic environments.
AB - High-yielding and selective prebiotic syntheses of RNA and DNA nucleotides involve UV irradiation to promote the key reaction steps and eradicate biologically irrelevant isomers. While these syntheses were likely enabled by UV-rich prebiotic environment, UV-induced formation of photodamages in polymeric nucleic acids, such as cyclobutane pyrimidine dimers (CPDs), remains the key unresolved issue for the origins of RNA and DNA on Earth. Here, we demonstrate that substitution of adenine with 2,6-diaminopurine enables repair of CPDs with yields reaching 92%. This substantial self-repairing activity originates from excellent electron donating properties of 2,6-diaminopurine in nucleic acid strands. We also show that the deoxyribonucleosides of 2,6-diaminopurine and adenine can be formed under the same prebiotic conditions. Considering that 2,6-diaminopurine was previously shown to increase the rate of nonenzymatic RNA replication, this nucleobase could have played critical roles in the formation of functional and photostable RNA/DNA oligomers in UV-rich prebiotic environments.
U2 - 10.1038/s41467-021-23300-y
DO - 10.1038/s41467-021-23300-y
M3 - Article
C2 - 34021158
AN - SCOPUS:85106564592
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3018
ER -