3 dimensional modelling of early human brain development using optical projection tomography

Janet Kerwin, Mark Scott, James Sharpe, Luis Puelles, Stephen C Robson, Margaret Martínez-de-la-Torre, Jose Luis Ferran, Guangjie Feng, Richard Baldock, Tom Strachan, Duncan Davidson, Susan Lindsay

Research output: Contribution to journalArticlepeer-review

Abstract

As development proceeds the human embryo attains an ever more complex three dimensional (3D) structure. Analyzing the gene expression patterns that underlie these changes and interpreting their significance depends on identifying the anatomical structures to which they map and following these patterns in developing 3D structures over time. The difficulty of this task greatly increases as more gene expression patterns are added, particularly in organs with complex 3D structures such as the brain. Optical Projection Tomography (OPT) is a new technology which has been developed for rapidly generating digital 3D models of intact specimens. We have assessed the resolution of unstained neuronal structures within a Carnegie Stage (CS)17 OPT model and tested its use as a framework onto which anatomical structures can be defined and gene expression data mapped.
Original languageEnglish
Pages (from-to)27
JournalBMC Neuroscience
Volume5
DOIs
Publication statusPublished - 6 Aug 2004

Keywords

  • Software
  • Imaging, Three-Dimensional
  • Humans
  • Gestational Age
  • Computer Graphics
  • Brain
  • Tomography
  • Models, Neurological

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