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Abstract / Description of output
Ovulation-associated inflammation with accompanied cytokines and reproductive hormones impact upon the human ovarian surface epithelium (hOSE) and probably have a role in the aetiology of ovarian cancer. Progesterone and progestin-related events, i.e. pregnancy and oral contraception, protect from the disease. We have investigated the pre-receptor metabolism of progesterone in primary hOSE cells and an immortalised hOSE cell line, OSE-C2, focusing on transcriptional regulation of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) by inflammatory, anti-inflammatory and apoptotic factors. In hOSE cells, we show that anti-inflammatory effects of IL-1alpha and IL-4 on 3beta-HSD2 mRNA involve a p38 MAPK signalling pathway, whereas pro-inflammatory response of IL-1alpha to 3beta-HSD1 mRNA involves a NF-kappaB inflammatory pathway. In OSE-C2 cells, retinoic acid and transforming growth factor-beta1 massively induce 3beta-HSD1 mRNA levels. In conclusion, we elaborate several mechanisms for intracrine formation of progesterone in hOSE that could contribute in the development of novel strategies to prevent, diagnose and/or treat ovarian cancer.
Original language | English |
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Pages (from-to) | 65-73 |
Number of pages | 9 |
Journal | Molecular and Cellular Endocrinology |
Volume | 301 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 25 Mar 2009 |
Keywords / Materials (for Non-textual outputs)
- Human ovarian surface epithelium
- OSE-C2 cells
- 3β-HSD
- Progesterone
- Inflammation
- Ovulation
- Epithelial ovarian cancer
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- 1 Finished
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Local Cortisol (CORT) Regulation in Ovarian and Uterine Disorders
1/10/05 → 31/10/20
Project: Research