In this paper we present our study regarding changes of human red blood cells (RBCs) immersed in solutions with different tonicity, monitored using digital holographic microscopy in order to connect their dimensions with the deformation states. We started with experimental images acquisition followed by our image processing procedure, which simultaneously provides information collected from more than one hundred cells from several images based on data from single cell level, and outputs are histograms after the main cell characteristics: minor radius, eccentricity, sphericity coefficient linked with the deformation states on three axes. It is also discussed how different digital filters are used for a better identification of the center and border of each cell.
|Number of pages||8|
|Journal||Proceedings of the Romanian Academy, Series A|
|Publication status||Published - 2013|