A 16S rRNA gene and draft genome database for the murine oral bacterial community

Susan Joseph, Joseph Aduse-Opoku, Ahmed Hashim, Eveliina Hanski, Ricarda Streich, Sarah C. L. Knowles, Amy B. Pedersen, William G. Wade, Michael A. Curtis, Nicola Segata (Editor)

Research output: Contribution to journalArticlepeer-review

Abstract

A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology.
Original languageEnglish
Article numbere01222-20
Number of pages15
JournalmSystems
Volume6
Issue number1
Early online date9 Feb 2021
DOIs
Publication statusE-pub ahead of print - 9 Feb 2021

Keywords

  • 16S rRNA
  • streptococcus danieliae
  • apodemus sylvaticus
  • database
  • mouse models
  • mus musculus
  • oral microbiology
  • oral microbiome

Fingerprint Dive into the research topics of 'A 16S rRNA gene and draft genome database for the murine oral bacterial community'. Together they form a unique fingerprint.

Cite this