A 19-year-old man with leucocyte adhesion deficiency. In vitro and in vivo studies of leucocyte function.

K A Davies, VJ Toothill, John C Savill, N Hotchin, A.M Peters, J D Pearson, Christopher Haslett, M. Burke, S.K Law, N F Mercer, M J Walport, A D Webster

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We describe a male patient with leucocyte adhesion molecule deficiency (LAD) of moderate phenotype. Although diagnosis was made only 2 years before his death, the patient survived until 19 years of age. This enabled us to perform a number of novel investigation, both in vivo and in vitro, relating to his leucocyte biology. Monocytes cultured in vitro matured into morphologically normal, phagocytically capable macrophages, which were able to recognize aged 'apoptotic' neutrophils. By injection of radiolabelled autologous neutrophils we demonstrated a prolonged neutrophil half-life, but normal margination, de-margination on exercise, and splenic pooling. Neutrophil adherence in vitro to vascular endothelium was normal. Histological examination of the patient's lungs at post-mortem showed intravascular aggregation of polymorphonuclear leucocytes but a paucity of cells in the interstitium and alveolar spaces. These findings indicate that the peripheral blood leucocytosis commonly observed in these patients may be due to prolonged intravascular neutrophil survival, and suggest that CD11/18 molecules have an important role in facilitating neutrophil emigration from blood vessels at sites of inflammation.
Original languageEnglish
Pages (from-to)223-31
Number of pages9
JournalClinical & Experimental Immunology
Volume84
Issue number2
Publication statusPublished - 1991

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